نوع مقاله : مقاله کامل پژوهشی
نویسندگان
1 کارشناسی ارشد، گروه مهندسی پزشکی، دانشگاه صنعتی امیرکبیر، تهران، ایران
2 استادیار، گروه مهندسی پزشکی، دانشگاه صنعتی امیرکبیر، تهران، ایران
3 استادیار، مرکز تحقیقات فناوری‌های نو دانشگاه صنعتی امیرکبیر، تهران، ایران
4 دانشجوی دکتری، گروه مهندسی پزشکی، دانشگاه صنعتی امیرکبیر، تهران، ایران
چکیده
بافت پوست اولین سد دفاعی بدن محسوب می‌شود. این بافت، به‌دلیل این‌که سطحی‌ترین بافت بدن است، براثر حوادث متعددی دچار آسیب می‌شود. یکی از ابزارهایی که در پزشکی از آن برای اتصال پیوندهای پوستی استفاده می‌شود منگنه‌های پوستی است. هدف این پژوهش ساخت منگنهٔ پلیمری تخریب‌پذیر پوستی است که بدین‌منظور از روش الکتروریسی به‌عنوان روش جایگزین اکسترودکردن و تزریق در قالب بهره برده شده است. از آزمون‌های فیزیکوشیمیایی برای بررسی خواص الیاف ساخته‌شده استفاده شد. نانوذرات سیلیکای SBA_15 (Santa Barbara Amorphous-15) ساخته‌شده با روش هیدروترمال به‌منظور بهبود خواص مکانیکی الیاف پلیمری اضافه شد. آزمون FTIR برای تعیین حضور نانوذرات در پلیمر به کار برده شد. آزمون BET برای بررسی اندازهٔ حفرات نانوذرات سیلیکای SBA_15 انجام شد و اندازهٔ تخلخل‌ها 77/6 نانومتر به دست آمد که تأییدی بر مزومتخلخل‌بودن نانوذرات است. برای بررسی ریخت‌شناسی الیاف الکتروریسی‌شده و نانوذرات سیلیکای ساخته‌شده، از تصویربرداری میکروسکوپ الکترونی روبشی SEM استفاده شد که مشخص شد میانگین قطر الیاف در محدودهٔ ۴۰۰-۵۰۰ نانومتر و میانگین قطر نانوذرات 18۵ نانومتر است. نتایج حاصل از افزودن نانوذرات سیلیکای SBA_15 به الیاف پلیمری و مقایسهٔ خواص مکانیکی آن با الیاف پلیمری PLGA خالص حاکی از آن بود که افزودن یک درصد نانوذرات سیلیکای SBA_15 به پلیمر از الیاف پلیمری خالص و الیاف دارای 3 درصد نانوذرات سیلیکای SBA_15 خواص مکانیکی بهتری را نشان داده است. هیچ‌یک از گروه‌های آزمون در رشد و تکثیر سلول‌ها ممانعتی ایجاد نکرد.
کلیدواژه‌ها
- منگنهٔ زیست‌تخریب‌پذیر پوستی
- کو پلیمر پلی‌لاکتیک/گلایکولیک اسید
- نانوذرات سیلیکا SBA_15
- آسکوربیک اسید
موضوعات
عنوان مقاله [English]
Synthesis and Characterization of Polylactic/Glycolic Acid Copolymer to Prepare Biodegradable Skin Staple
نویسندگان [English]
- Zeynab Bateni 1
- Mahnaz Eskandari 2
- Masoumeh Haghbin Nazarpak 3
- Shadi Askari 4
1 Master, Department of Biomedical Engineering, Amirkabir University of Technology (AUT), Tehran, Iran.
2 Assistant Professor, Department of Biomedical Engineering, Amirkabir University of Technology (AUT), Tehran, Iran.
3 Assistant Professor, New Technologies Research Center (NTRC), Amirkabir University of Technology (AUT), Tehran, Iran.
4 PhD Student, Department of Biomedical Engineering, Amirkabir University of Technology (AUT), Tehran, Iran.
چکیده [English]
Skin tissue serves as the body’s first defense barrier. Since this tissue is the most superficial one in the body, it is susceptible to damages from many incidents. Skin staples are one of the tools used in medicine to connect skin grafts. The purpose of this research is to make a degradable polymer skin stapler that uses electrospinning as an alternative to extruding and injecting into a mold. Physicochemical tests were done to determine the properties of the manufactured PLGA fibers. Silica nanoparticles SBA-15 made by hydrothermal method were added to improve the mechanical properties of polymer fibers. Then, FTIR test was carried out to detect the presence of nanoparticles in the polymer. The BET test was also performed to measure the size of the pores of SBA-15 silica nanoparticles as 6.77 nm, which is a confirmation of the mesoporous nature of the nanoparticles. To investigate the morphology of the electrospun fibers and silica nanoparticles, SEM image was used according to which, the average diameter of fibers and average diameter of nanoparticles were obtained as 400-500 nm and 185 nm, respectively. The results obtained from addition of SBA_15 silica nanoparticles to polymer fibers and those from comparing its mechanical properties with pure PLGA polymer fibers indicated that incorporating 1% SBA_15 silica nanoparticles into polymer improved its mechanical properties over pure polymer fibers, while fibers with 3% Silica nanoparticles showed SBA_15 comparable results. According to cytotoxicity investigation, no toxicity was observed in any group, and all groups supported the cell growth.
کلیدواژه‌ها [English]
- Biodegradable Skin Stapler
- Co-Polymer Polylactic/Glycolic Acid
- Silica Nanoparticles SBA_15
- Ascorbic Acid
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