نوع مقاله : مقاله کامل پژوهشی
نویسندگان
1 دانشکده داروسازی و علوم دارویی، دانشگاه علوم پزشکی آزاد اسلامی، تهران، ایران
2 دانشکده علوم پایه، واحد ورامین- پیشوا، دانشگاه آزاد اسلامی، ورامین، ایران
چکیده
در این پژوهش، بیونانوکامپوزیت گرافن اکسید پیوندخورده با یک پلیمر مشتق از سلولز طبیعی (CCG) تهیه شد و کارایی آن به عنوان یک حامل هدفمند برای بارگذاری داروی ضدسرطان کورکومین مورد مطالعه قرار گرفت. ویژگی‌های سطحی و ساختاری CCG با آنالیزهای FESEM، BET، و FTIR بررسی شد. روش سطح پاسخ (RSM) و طرح کامپوزیت مرکزی برای بهینه‌سازی عوامل مؤثر شامل pH، زمان تماس، و غلظت اولیه دارو بر درصد جذب دارو روی کامپوزیت استفاده شد. نتایج حاصل از طراحی کامپوزیت مرکزی نشان داد که یک مدل درجه دوم با ضریب تعیین 9845/0 و 0001/0 > p برای برازش داده‌های تجربی مناسب است و بیشینه جذب (70 درصد) در شرایط 6 = pH، زمان تماس 60 دقیقه و غلظت اولیه 20 میلی‌گرم بر لیتر پیش‌بینی شد. درصد رهایش دارو از نانوحامل CUR@CCG در6/5 = pH به میزان چشمگیری بیش از 4/7 = pH بود که نشان دهنده اثربخشی سامانه در محیط سلول سرطانی است. اثر سمیت سلولی CUR، CCG، و CUR@CCG روی دو رده سلولی سالم (MCF 10A) و سرطانی (MDA-MB 231) پستان به روش MTT بررسی شد. اثر سمیت سلولی CUR@CCG در غلظت 96 میکروگرم بر میلی‌لیتر و در زمان 24 ساعت بر سلول‌های MDA-MB 231، در حدود 45 درصد و کمی کمتر از CUR آزاد (50 درصد) بود. بر اساس نتایج حاصل، بیونانوکامپوزیت CCG می‌تواند به عنوان یک نانوحامل زیست‌سازگار و هدفمند برای داروی ضد سرطان CUR مورد استفاده قرار گیرد.
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
Design of a Polymer-grafted Graphene Oxide Based Drug Delivery System for Curcumin: Optimization Using RSM and Cytotoxicity study
نویسندگان [English]
- Elham Daneshmoghanlou 1
- Mahsasadat Miralinaghi 2
- Elham Moniri 2
1 School of Pharmacy and Pharmaceutical Sciences, Islamic Azad University of Medical Sciences, Tehran, Iran
2 Department of Chemistry, Faculty of Science, Varamin - Pishva Branch, Islamic Azad University,
چکیده [English]
In the present research, cellulose-conjugated graphene oxide (CCG) was prepared, and its efficiency as a targeted carrier was explored for loading curcumin, an anti-cancer drug. The structural and surface properties of CCG were examined by FESEM, BET, and FTIR techniques. Response surface method (RSM) with the central composite design was employed to optimize the effective parameters, such as pH, contact time, and initial drug concentration, on the drug adsorption on the composite. The central composite design results indicated that a second-order equation can properly fit the experimental data with a coefficient of determination of 0.9845 and p < 0.0001. The maximum adsorption (70%) was achieved at pH = 6, contact time of 60 min, and initial concentration of 20 mg L−1. The drug release from the CUR@CCG nanocarrier at pH = 5.6 was significantly higher than pH = 7.4, demonstrating the effectiveness of the system in the cancer cell environment. The MTT assay was used to assess the cytotoxicity of CUR, CCG, and CUR@CCG on two cell lines, including normal (MCF 10A) and breast cancer (MDA-MB 231). The cytotoxicity of CUR@CCG at a concentration of 96 μg mL−1 for 24 h on MDA-MB 231 was almost 45%, slightly lower than free CUR (50%). Based on these results, the CCG bio-nanocomposite can be utilized as a biocompatible nanocarrier for targeted delivery of the CUR anti-cancer agent.
کلیدواژه‌ها [English]
- polymer composite
- central composite design
- cell viability
- targeted drug delivery
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