نوع مقاله : مقاله کامل پژوهشی
نویسندگان
گروه مهندسی مواد، دانشکده فنی و مهندسی، دانشگاه بین المللی امام خمینی (ره)، قزوین، قزوین، ایران
چکیده
این پژوهش، با هدف بررسی تأثیر حضور مس و منیزیم بر خواص ساختاری، زیستی و عملکرد ضدباکتریایی شیشه های زیست فعال 58S انجام شده است. ارزیابی ساختاری و ریزساختاری سطح شیشه های سنتز شده به روش سل-ژل، با استفاده از آزمون های پراش اشعه XRD) X)، طیف‌سنجی تبدیل فوریه مادون قرمز (FTIR) و میکروسکوپ الکترونی روبشی (SEM) صورت گرفت. تأثیر ترکیب مس و منیزیم بر کیفیت و کمیت زیست فعالی برون تنی، به کمک آزمون های زولیم برماید (MTT) و آلکالین فسفاتاز (ALP) مورد بررسی قرار گرفت. عملکرد ضدباکتریایی شیشه های زیست فعال حاوی مس و منیزیم در برابر باکتری MRSA، بررسی شد. نتایج، تشکیل لایه هیدروکسی آپاتایت را روی سطح شیشه ی زیستفعال تأیید کرد. افزودن مس و منیزیم به ترکیب شیشه زیست فعال 58S، موجب افزایش فعالیت و تکثیر سلولی و بهبود عملکرد ضدباکتریایی شد. نتایج حاکی از مقدار بهینه منیزیم و مس در ترکیب شیشه زیست فعال BG-5/5 (حاوی 5 درصد مولی از هر کدام از MgO و CuO) است.
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
Investigation the In Vitro and Bactericidal Properties of Magnesium and Copper Containing Bioactive Glasses
نویسندگان [English]
- Amirhossein Moghanian
- Mohammad Amin Zohour Fazeli
Department of Materials Engineering, Imam Khomeini International University, Qazvin, Qazvin, Iran
چکیده [English]
The study aimed to investigate the performance and evaluate the effect of incorporation of Cu and Mg on the structural, biological, and bactericidal properties of the sol-gel derived 58S bioactive glass containing Mg and Cu. The structural and morphological evaluations of the synthesized glasses were performed by the mean of X-ray diffraction (XRD), Fourier transforms infrared spectroscopy (FTIR), and scanning electron microscopy (SEM) analysis. The effect of Cu and Mg content in the composition on the quality and quantity of in vitro bioactivity was examined by performing 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) and alkaline phosphate (ALP) analysis. The antibacterial performance of Cu and Mg incorporated bioactive glasses were evaluated against MRSA bacteria. The results confirmed the formation of a hydroxyapatite layer on the glass surface. Adding copper and magnesium to the 58S bioactive glass composition increased cell activity and proliferation and improved antibacterial performance. The results suggest that BG-5/5 (including 5 mol % of both CuO and MgO) as the bioactive glass with the optimal amount of magnesium and copper in its composition.
کلیدواژه‌ها [English]
- Apatite
- Bioactivity
- Biomedical applications
- Sol-gel process
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