In the current study, the effect of performing the thermal cycling on the impact behavior of Fiber Metal Laminates (FMLs) fabricated by aluminum and carbon fibers/epoxy composites was investigated. To this end, first, the FMLs with various configurations of carbon fibers (0˚/0˚/0˚/0˚, 90˚/90˚/90˚/90˚, +30˚/-30˚/-30˚/+30˚, and 0˚/90˚/90˚/0˚) were fabricated and put into oven under the thermal cycling conditions with 0, 1, 10, 30, 50, 70, and 90 cycles. For each thermal cycle, the samples were put into the oven for 15 min to increase their temperature from the ambient temperature to 100 °C. Then, the temperature was kept constant for five minutes. Next, FMLs were extracted from the oven to reduce their temprature by the ambient temperature for 15 min. Then, the impact behavior of these samples was investigated based on Charpy impact test, and the related mechanisms were characterized by macrostructural and microstructural methods. The obtained results confirmed that followed by the thermal cycling on the unidirectional samples (0˚/0˚/0˚/0˚ and 90˚/90˚/90˚/90˚), the absorbed energy capability was considerably improved. To be specific, the maximum improvement values in these configurations were obtained as 21.4 and 19.4 %, respectively. It was also found that by changing the configurations, the enhancement and reduction of the absorbed energy capability were transferred in the higher thermal cycles. The microstructural investigations revealed that after thermal cycles, the fracture behavior was altered, and the adhesion between carbon fibers and epoxy matrix was deteriorated.