مواد و فناوری‌های پیشرفته

مواد و فناوری‌های پیشرفته

ساخت و بررسی خواص داربست فیبروئین ابریشم حاوی نانوذرات کیتوسان بارگذاری‌شده با آسکوربیک اسید به‌منظور کاربرد در بازسازی استخوان

نوع مقاله : مقاله کامل پژوهشی

نویسندگان
پگاه سنجرنیا 1
ژامک نورمحمدی 2
علی حسین رضایان 2
مهرناز مؤدب 1
1 دانشجوی کارشناسی ارشد، دانشکده علوم و فنون نوین، دانشگاه تهران، تهران، تهران، ایران
2 دانشیار، دانشکده علوم و فنون نوین، دانشگاه تهران، تهران، تهران، ایران
10.30501/jamt.2021.266866.1152
چکیده
هدف از این پژوهش، ساخت و بررسی خواص داربست فیبروئینی حاوی نانوذرات کیتوسان بارگذاری شده با آسکوربیک اسید بود. برای این منظور، نانوذرات آسکوربیک اسید-کیتوسان به روش ژل‌شدن یونی ساخته شدند. تصاویر میکروسکوپ الکترونی روبشی (SEM) و نتایج پراکندگی نور دینامیکی (DLS) نشان دادند که نانوذرات کروی‌شکل دارای ذراتی با اندازه میانگین 200 نانومتر هستند. سپس، مقادیر مختلفی از نانوذرات در داخل محلول فیبروئین ابریشم قرار گرفت و داربست‌های مورد‌نظر به روش خشکایش انجمادی تهیه شدند. تأثیر غلظت‌های مختلف نانوذرات بر ویژگی‌های ریخت‌شناسی، تغییرات ساختاری، جذب آب، رهایش دارو، سمّیت، چسبندگی و فعالیت آلکالین فسفاتاز سلول‌های استئوسارکومای رد‌ه MG63، مطالعه و بررسی شد. نتایج به‌دست‌آمده از طیف‌سنجی تبدیل فوریه فروسرخ (FTIR)، وجود نانوذرات در داربست را تأیید کرد. بررسی‌ ریخت‌شناسی سطح مقطع داربست‌ها نشان داد که همه داربست‌ها دارای ساختاری متخلخل با حفره‌های به‌هم‌متصل هستند. افزایش نانوذرات سبب کاهش اندازه قطر حفره‌ها و درصد تخلخل شد. رهایش آسکوربیک اسید در همه نمونه‌ها، با رهایش انفجاری در ۲۴ ساعت اولیه، شروع شد و سپس به صورت کنترل شده تا ۱۴ روز ادامه یافت. مقدار رهایش آسکوربیک اسید  با افزایش درصد نانو ذرات در داربست افزایش یافت. بررسی‌های سلولی نشان داد که داربست، دارای سمّیت نیست و سلول‌های MG63 به‌خوبی به سطح داربست‌ها و دیواره حفره‌ها می‌چسبند. همچنین تکثیر و فعالیت آلکالین فسفاتاز سلول‌های MG63، با افزایش مقدار آسکوربیک اسید، افزایش می‌یابد.
کلیدواژه‌ها
آسکوربیک اسید
داربست استخوانی
فیبروئین ابریشم
نانوذرات کیتوسان

موضوعات

عنوان مقاله English

Fabrication and Characterization of Properties of Silk Fibroin Scaffold Containing Ascorbic Acid-Loaded Chitosan Nanoparticles for Bone Regeneration Applications

نویسندگان English

Pegah Sanjarnia 1
Jhamak Nourmohammadi 2
Ali Hossein Rezayan 2
Mehrnaz Moaddab 1
1 M. Sc. Student, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Tehran, Iran
2 Associate Professor, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Tehran, Iran
چکیده English

The aim of this study was to fabricate and characterize silk fibroin scaffold containing chitosan nanoparticle loaded with ascorbic acid. Therefore, ascorbic acid- chitosan nanoparticles were fabricated using the ionic gelation method. Scanning Electron Microscopy (SEM) images and Dynamic Light Scattering (DLS) results showed that the nanoparticles are spherical with the average size of 200 nm. Then, different amounts of nanoparticles were placed in the silk fibroin solution, and finally, the scaffolds were prepared by the freeze-drying method. The effect of nanoparticle concentrations on various properties such as morphology, structural changes, water absorption, drug release, toxicity, adhesion, and alkaline phosphatase activity of MG63 cells were studied. The results of Fourier Transform Infrared Spectroscopy (FTIR) confirmed the presence of nanoparticles in the scaffold. Morphological examinations of the cross-section of the scaffold showed that all scaffolds have a porous structure with interconnected pores. The mean size of the pores and porosity percentages reduced as the nanoparticle content rose. The release of ascorbic acid in all samples started with the burst release in the first 24 hours and then continued with a controlled release for up to 14 days. Higher amounts of ascorbic acid were released from the scaffold containing more nanoparticles. Cellular studies showed that the scaffold was non-toxic and that MG63 cells adhered well onto the surface of the scaffold and the pores’ wall. Also, the proliferation and alkaline phosphatase activity of MG63 cells increased with increasing ascorbic acid amounts.

کلیدواژه‌ها English

Ascorbic Acid
Bone Scaffold
Silk Fibroin
Chitosan Nanoparticles
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مواد و فناوری‌های پیشرفته
دوره 10، شماره 4
زمستان 1400
صفحه 25-36

  • تاریخ دریافت 21 بهمن 1399
  • تاریخ بازنگری 11 اردیبهشت 1400
  • تاریخ پذیرش 16 اردیبهشت 1400