نوع مقاله : مقاله کامل پژوهشی

نویسندگان

پژوهشکده فناوری نانو و مواد پیشرفته، پژوهشگاه مواد و انرژی، مشکین دشت، البرز، ایران

10.30501/jamt.2021.214580.1070

چکیده

بسپارهای تخریب ­پذیر زیستی با ماهیت غیرسمّی و زیست­ سازگار، در مهندسی بافت و رهایش دارو کاربرد دارند. مصرف ریزذرات بسپار طبیعی، به‌صورت مستقل یا در ترکیب با مواد دیگر، با کاربردهای گوناگون ازجمله زیست ­مواد تزریق­ پذیر، در حال گسترش است. در این پژوهش، برهم‌کنش بسپارهای زیستی ژلاتین و سدیم ­آلژینات در ساخت ریزکرات، مطالعه شد. برهم‌کنش این دو بسپار، متأثّر از pH، بار الکتریکی و غلظت آن‌ها است و واکنش الکترواستاتیک بین پروتئین ­های با بار مثبت و پلی ­ساکاریدهای آنیونی، منجر به تشکیل فازهای محلول و نامحلول می­ شود؛ بنابراین، تأثیر نسبت مخلوط کلوئیدی و pH، در هم­ انباشتی بررسی گردید. در ابتدا، وزن مولکولی ژلاتین با روش حلال ­زدایی، باریک شد و رنگ ­نگاری ژل ­تراوایی (GPC)، خروج ژلاتین با وزن مولکولی کم را تأیید کرد. بررسی مایع شناور مخلوط­ های کلوئیدی با آزمون طیف ­سنجی مرئی فرابنفش (UV-Vis)، نشان داد که هم­ انباشتی بهینه، در نسبت ژلاتین به سدیم ­آلژینات برابر چهار و pH برابر 65/3 رخ داده است. طی این فرایند، ریزکرات در اندازه 10 تا 25 میکرومتر با سطوحی صاف تشکیل شد و با افزایش مقدار جنیپین، تمایل به هم­نشینی ذرات مستقل ریزکرات بیشتر می ­شود. تغییر اندک ژلاتین در طیف دورنگ‌نمایی دورانی (CD)، برهم‌کنش با سدیم ­آلژینات را نشان می‌دهد و مشاهده گروه عاملی آمید در طیف ­سنجی فروسرخ تبدیل فوریه (FTIR) نیز، آن را تأیید می‌کند. نتایج دمانگاشت آزمون DSC، نشان‌دهنده­ افزایش دمای تخریب ریزکرات دارای مقادیر بیشتر جنیپین بوده است.

کلیدواژه‌ها

عنوان مقاله [English]

Preparation and Characterization of Microspheres Based on Gelatin-Sodium Alginate-Genipin by the Formation of Polyelectrolyte Complex

نویسندگان [English]

  • Majid Rastegar Ramsheh
  • Aliasghar Behnamghader
  • Ali khanlarkhani

Department of Nanotechnology and Advanced Materials, Materials and Energy Research Center (MERC), MeshkinDasht, Alborz, Iran

چکیده [English]

The Biodegradable polymers with non-toxic and biocompatible nature have applications in tissue engineering and drug delivery. The use of natural polymer microbeads independently or in combination with other materials is gaining ground in various applications including injectable biomaterials. In this research, the interaction of gelatin and sodium alginate biopolymers was investigated in the manufacture of microsphere. The interaction between these two polymers was influenced by their pH, charge, and concentration. The electrostatic reaction between positively charged proteins and anionic polysaccharides led to the formation of soluble and insoluble phases. Therefore, the effect of pH and colloidal ratio was examined on the Coacervation. First, gelatin molecular weight was narrowed by Desolvation method, and GPC confirmed the elimination of low molecular weight gelatin. The supernatants of colloidal mixtures were analyzed by UV-Vis test, and the results revealed that optimum coacervation occurred at a gelatin/sodium alginate ratio of four and a pH value of 3.65. During this process, microsphere formed with smooth surfaces in a size range of 10-25 µm. An increase in the amount of genipin raised the aggregation tendency in independent microbeads. A slight change of gelatin in Circular Dichroism (CD) reflected the interaction with sodium alginate, which was confirmed by the amide functional group in the Fourier-transform infrared (FTIR) spectroscopy. Results of Thermogram of Differential Scanning Calorimetry (DSC) indicated an increase in the degradation temperature of microspheres containing higher amounts of genipin.

کلیدواژه‌ها [English]

  • Microsphere
  • Gelatin
  • Na Alginate
  • Genipin
  • Polyelectrolyte
  1.  

     

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