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ساخت و بررسی خواص فیزیکی و بیولوژیکی داربست‌‌های بر پایه هیدروکسی اتیل سلولز/هیالورونیک اسید مورد استفاده در ترمیم زخم‌های سوختگی درجه دوم سطحی

نوع مقاله : مقاله کامل پژوهشی

نویسندگان

1 پژوهشکده فناوری نانو و مواد پیشرفته، پژوهشگاه مواد و انرژی، مشکین دشت، البرز، ایران

2 گروه مهندسی بافت و علوم سلولی کاربردی، دانشکده فناوری‌های نوین پزشکی، دانشگاه علوم پزشکی تهران، تهران، تهران، ایران

چکیده

هدف از این مطالعه، تهیه داربست­‌هایی بر پایه هیدروکسی اتیل سلولز/هیالورونیک اسید، با و بدون حضور ژلاتین و مقایسه آنها، برای ترمیم زخم­ های سوختگی درجه دو سطحی است. داربست‌ها، از محلول آبی پلیمرهای ذکر شده و به روش خشک کردن انجمادی، تهیه شدند. بررسی ریزساختاری با میکروسکوپ الکترونی روبشی، نشانگر میانگین اندازه حفرات 120 و 98 میکرومتر، به ‌ترتیب برای داربست­ های فاقد ژلاتین و دارای ژلاتین بود. بر اساس نتایج رئولوژی، در تمامی نمونه ­ها، همواره مقدار مدول اتلاف، بیشتر از مدول ذخیره بود و محلول­های تهیه­ شده، جریان ­پذیر بودند. تمامی نمونه­ ها، در بسامدهای کم، دارای رفتار رقیق برشی و در بسامدهای بالاتر از s-1 100، رفتار عکس، از خود نشان دادند. با افزودن ژلاتین، اندازه هر دو مدول اتلاف و ذخیره، افزایش یافت. آزمون جذب مایع و کاهش وزن نمونه­‌ها، توانایی زیاد داربست­‌ها را در میزان جذب تا بالای 3000 درصد، نشان داد. افزودن ژلاتین، موجب افزایش زمان فروپاشی شبکه، تا 2 ساعت و کاهش نرخ تخریب داربست ­ها شد. آزمون سمّیت سلولی، نشانگر زنده­ مانی بیش از 80 درصد سلول­های فیبروبلاست مجاور نمونه ­ها (در مقایسه با نمونه­ های کنترل) بود؛ ضمن اینکه حضور ژلاتین، بر فعالیت متابولیکی سلول­ها، تأثیر مثبت داشت و موجب افزایش سرعت تکثیر سلول­‌ها شد. نتایج آزمون خراش، توانایی داربست­‌ها را در افزایش سرعت مهاجرت سلولی، نسبت به نمونه کنترل، نشان داد. داربست هیدروکسی اتیل سلولز/هیالورونیک اسید/ژلاتین، به‌علت حضور ژلاتین و تأثیر آن در افزایش بیشتر سرعت مهاجرت سلول­ها که نشانگر شکل­ گیری سریع بافت پوششی اپی تلیوم بود، انقباض زخم را طی 24 ساعت، به 73 درصد رساند.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Fabrication and Evaluation of Physical and Biological Properties of Hydroxyethyl Cellulose/Hyaluronic Acid-Based Scaffolds Used for Second-Degree (Partial-Thickness) Burns Wounds Healing

نویسندگان [English]

  • Atefe Derakhshani 1
  • Saeed Hesaraki 1
  • Nader Nezafati 1
  • Mahmoud Azami 2

1 Department of Nanotechnology and Advanced Materials, Materials and Energy Research Center (MERC), MeshkinDasht, Alborz, Iran

2 Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Tehran, Iran

چکیده [English]

The purpose of this study was to prepare and compare a hydroxyethyl cellulose/hyaluronic acid (HEC/HA)-based scaffold with and without gelatin for the treatment of second-degree partial-thickness burns. The scaffolds were prepared by freeze-drying method from the aqueous solutions of the mentioned polymers. Microstructural examination by scanning electron microscopy (SEM) indicated the average pore size of 120 and 98 µm for the gelatin-free and gelatin-containing scaffolds, respectively. According to the dynamic rheology measurements (DMA), all the solutions were flowable in which, the loss modulus was higher than the storage one. Moreover, the solutions revealed shear-thinning behaviour at low frequencies, which changed to shear thickening at frequencies higher than 100 s-1. Both loss and storage modulus increased by adding gelatin to the polymer solutions. The scaffolds water uptake was up to 3,000%. The addition of gelatin to the HA/HEC solution increased the polymer network collapse up to 2 hours and led to reducing the scaffolds degradation rate. Cytotoxicity assay in the presence of the scaffolds showed that more than 80 % of the fibroblast cells were viable (compared to the control group), meanwhile, the presence of  gelatin had a positive effect on the metabolic activity of the cells and increased the rate of cell proliferation.  Scratch test results showed the ability of scaffolds to increase the cell migration rate compared to the control sample. The hydroxyethyl cellulose/hyaluronic acid/gelatin scaffold due to the presence of gelatin and improved the cell migration, which indicating the rapid re-epithelialization, reduced wound contraction to   73 % within 24 hours.

کلیدواژه‌ها [English]

  • Hydroxyethyl Cellulose
  • Hyaluronic Acid
  • Gelatin
  • Burn Wound
  • Scaffold
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مواد و فناوری‌های پیشرفته
دوره 9، شماره 4
اسفند 1399
صفحه 35-46
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  • تاریخ دریافت: 24 فروردین 1399
  • تاریخ بازنگری: 19 آبان 1399
  • تاریخ پذیرش: 14 اسفند 1399
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