نوع مقاله : مقاله کامل پژوهشی

نویسندگان

گروه مهندسی مواد، دانشکده مهندسی معدن و متالورژی، دانشگاه یزد، یزد، یزد، ایران

10.30501/jamt.2020.221997.1075

چکیده

در این پژوهش، از روشی سریع، سازگار با محیط ­زیست و کم ­هزینه، برای تولید انبوه گرافن اکسید استفاده شده است. در این فرآیند، گرافن اکسید، از باگاس نیشکر خوزستان (به­ عنوان ضایعات زراعی)، طی عملیات حرارتی تک­ مرحله ای، در کوره مافلی، در دمای 350 درجه سلسیوس، به مدت 15 دقیقه، در محیط اکسایشی، سنتز شد. نتایج حاصل از آزمون ­های طیف ­سنجی تبدیل فوریه مادون قرمز و پراش پرتو ایکس، تشکیل گرافن اکسید را به ­خوبی تأیید می ­کند. نتایج بررسی ­های میکروسکوپ الکترونی روبشی و میکروسکوپ نیروی اتمی، نشان می ­دهد که میزان چین ­خوردگی ­های ورق­ه ای گرافن اکسید، کم، تعداد لایه ­ها، 5 تا 6 عدد، اندازه متوسط ضخامت صفحات، 5 نانومتر و متوسط ابعاد جانبی صفحات، 500 نانومتر است. آزمون سمّیت این ماده­ سنتز­شده، در غلظت ­های 200، 400 و 600 میکرو­گرم بر میلی ­لیتر محیط کشت باکتری، روی باکتری ­های گرم مثبت استافیلوکوکوس اورئوس، سویه گرم منفی اشریشیاکلی و سودوموناس آئروژینوزا، بررسی شد. نتایج، نشان می­ دهد که گرافن اکسید، در غلظت­ های 400 و 600 میکرو­گرم بر میلی­ لیتر، برای باکتری­ ها، نقش سمّی و ضد­باکتریایی داشته و میزان رشد باکتر ی­ها، پایین است. ه مچنین، نان وورقه ­های گرافن اکسید، اثر ضد­باکتری بیشتری نسبت به باکتری گرم مثبت، در مقایسه با باکتری­ های گرم منفی دارند. نتایج آنالیز حرارتی افتراقی روی باگاس نیشکر، در محیط هوا، نشان می­ دهد که شکل­ گیری گرافن اکسید، در محدوده دمایی 350 درجه سلسیوس بوده اما در محیط آرگون، این دما بالاتر است. به ­علاوه، نتایج آنالیز حرارتی انجام ­شده روی محصول گرافن اکسید سنتز­شده در محیط اکسیدی، نشان­ دهنده شروع ناپایداری آن از دمای 60 درجه سلسیوس است.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Evaluation of Microstructural and Antibacterial Properties of Graphene Oxide Synthesized by Green Method

نویسندگان [English]

  • Mojdeh Azizi
  • Mahdi Kalantar

Materials Engineering Group, Department of Mining and Metallurgical Engineering, Yazd University, Yazd, Yazd, Iran

چکیده [English]

In this research, a fast, environmentally friendly, and low-cost method has been used for the synthesis of large-scale production of graphene oxide. In this process, graphene oxide was synthesized via single-step reforming of sugarcane bagasse agricultural waste by oxidation under muffled atmosphere conditions at 350 ºC and for 15 minutes. The results of Fourier transform infrared spectroscopy and X-ray diffraction analyses confirm the formation of graphene oxide. The results of scanning electron microscopy and atomic force microscopy studies show that the graphene oxide sheets are with few wrinkles, the number of layers is 5-6 layers, the average size of the plate thickness is 5 nm, and the average lateral dimensions of the plates is 500 nm. The synthesized graphene oxide was evaluated by the toxicity test at concentrations of 200, 400, and 600 μg/ml on Gram-positive bacteria, “Streptococcus iniae”, and Gram-negative bacteria, “Escherichia coli” and “Pseudomonas aeruginosa”. The results show that graphene oxide at concentrations of 400 and 600 μg / ml has a toxic and an antibacterial agent for bacteria and it has reduced the growth rate of bacteria. In addition, graphene oxide nanosheets are more toxic to gram-positive bacteria than gram-negative bacteria. The results of differential thermal analysis on sugarcane bagasse show that the formation of graphene oxide in air is at a temperature of 350 °C but it is in argon atmosphere is higher. In addition, the results of the thermal analysis performed on the synthesized graphene oxide in the air atmosphere indicate that its instability begins at 60 °C.

کلیدواژه‌ها [English]

  • graphene oxide
  • Synthesis
  • Green method
  • antibacterial properties

 

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