نوع مقاله : مقاله کامل پژوهشی
نویسندگان
1 پژوهشگر، دانشکده مهندسی مواد و متالورژی، دانشگاه علم و صنعت ایران، تهران، تهران، ایران
2 استاد، دانشکده مهندسی مواد و متالورژی، دانشگاه علم و صنعت ایران، تهران، تهران، ایران
چکیده
در این پژوهش، هیدروژل‌های ژلاتین/کیتوسان/اکسید روی به‌روش ریخته‌گری محلول پلیمری در ترکیب با روش خشک‌کردن انجمادی تهیه و تأثیر افزودن 5/1 درصد وزنی نانوذرات اکسید روی بر ویژگی‌های ریزساختاری و فیزیکی ـ شیمیایی داربست‌های کراس‌لینک شده یا 5/1 درصد وزنی جنیپین مطالعه شد. آنالیز تبدیل فوریه مادون قرمز نشان‌دهنده برهم‌کنش‌های فیزیکی پلیمر و اکسید روی بود. با افزودن اکسید روی، میزان تخلخل هیدروژل‌ها از حدود 93 به 94 درصد افزایش یافت (05/0 > P). تصاویر میکروسکوپ الکترونی روبشی (SEM) داربست‌های نانوکامپوزیتی، نشان‌دهنده تشکیل ریزساختار متخلخلی از حفرات مشخص به‌هم‌پیوسته با متوسط اندازه حفرات 200 میکرون بود. توانایی جذب آب هیدروژل‌های ژلاتین/کیتوسان در دمای اتاق و دمای 37 درجه سلسیوس با افزودن اکسید روی، به‌ترتیب، از 1043 به 988 و از 1206 به 1040درصد کاهش یافت، اما افزایش قابل‌توجهی در سرعت تورم اولیه آن‌ها دیده شد. با افزودن نانوذرات، تخریب برون‌تنی داربست‌ها سریع‌تر رخ داد. با توجه به یافته‌های این پژوهش، هیدروژل‌های ژلاتین/کیتوسان/اکسید روی، که ویژگی‌های ریزساختاری مطلوب (یعنی، توزیع یکنواختی از حفرات به‌هم‌پیوسته) و سرعت تورم اولیه بالایی دارد، به‌منزله بستری بالقوه برای استفاده در سامانه‌های دارویی یا حوزه مهندسی بافت پیشنهاد می‌شوند.
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
Fabrication and Characterization of Gelatin/Chitosan/Zinc Oxide Nanocomposite Hydrogels Intended for Biomedical Applications
نویسندگان [English]
- Shadi Moshayedi 1
- Hossein Sarpoolaky 2
- Alireza Khavandi 2
1 Researcher, School of Materials and Metallurgical Engineering, Iran University of Science and Technology, Tehran, Tehran, Iran
2 Professor, School of Materials and Metallurgical Engineering, Iran University of Science and Technology, Tehran, Tehran, Iran
چکیده [English]
In the present study, gelatin/chitosan/zinc oxide hydrogels were prepared through solvent casting method in combination with lyophilization. In addition, the effects of adding 1.5 wt % zinc oxide nanoparticles on the microstructural and physico-chemical characteristics of genipin-crosslinked scaffolds were evaluated. The porosity of the newly formed hydrogels increased from about 93 up to 94 % (P < 0.05). Images taken by a Scanning Electron Microscope (SEM) illustrates the formation of a porous microstructure of distinct interconnected holes with an average size of 200 microns. Water absorption capacity of nanocomposite hydrogels at room temperature and 37 °C decreased from 1043 to 988 and 1206 to 1040 %, respectively; however, a significant increase in their initial swelling rate was observed. Upon adding nanoparticles, the in vitro degradation of scaffolds occurred faster than usual. According to the findings of this research, gelatin/chitosan/zinc oxide hydrogels which is characterized by favorable microstructural characteristics (i.e., uniform distribution of interconnected pores) and high initial swelling rate can be used as a potential substrate in the field of tissue engineering.
کلیدواژه‌ها [English]
- Hydrogel
- Gelatin
- Chitosan
- Zinc Oxide
- El-Sherbiny, I. M., Yacoub, M. H., "Hydrogel scaffolds for tissue engineering: Progress and challenges", Global Cardiology Science & Practice, 2013, No. 3, (2013), 316-342. https://doi.org/10.5339/gcsp.2013.38
- Li, J., Mooney, D. J., "Designing hydrogels for controlled drug delivery", Nature Reviews Materials, Vol. 1, No. 12, (2016), 16071, 1-17. https://doi.org/10.1038/natrevmats.2016.71
- Narayanaswamy, R., Torchilin, V. P., "Hydrogels and their applications in targeted drug delivery", Molecules, Vol. 24, No. 3, (2019), 603, 1-21. https://doi.org/10.3390/molecules24030603
- Sultana, N., Hassan, M. I., Ridzuan, N., Ibrahim, Z., Soon, C. F., "Fabrication of gelatin scaffolds using thermally induced phase separation technique", International Journal of Engineering, Vol. 31, No. 8, (2018), 1302-1307. http://doi.org/10.5829/ije.2018.31.08b.19
- Bello, A. B., Kim, D., Kim, D., Park, H., Lee, S. H., "Engineering and functionalization of gelatin biomaterials: From cell culture to medical applications", Tissue Engineering Part B: Reviews Revision, Vol. 26, No. 2, (2020), 164-180. https://doi.org/10.1089/ten.TEB.2019.0256
- Yang, G., Xiao, Z., Long, H., Ma, K., Zhang, J., Ren, X., Zhang, J., "Assessment of the characteristics and biocompatibility of gelatin sponge scaffolds prepared by various crosslinking methods", Scientific Reports, Vol. 8, No. 1, (2018), 1-13. https://doi.org/10.1038/s41598-018-20006-y
- Song, Y., Nagai, N., Saijo, S., Kaji, H., Nishizawa, M., Abe, T., "In situ formation of injectable chitosan-gelatin hydrogels through double crosslinking for sustained intraocular drug delivery", Materials Science & Engineering: C, Materials for Biological Applications, Vol. 88, (2018), 1-12. https://doi.org/10.1016/j.msec.2018.02.022
- Vlăsceanu, G., Crica, L., Pandele, A., Ionita, M., "Graphene oxide reinforcing genipin crosslinked chitosan-gelatin blend films", Coatings, Vol. 10, No. 2, (2020), 189, 1-15. https://doi.org/10.3390/coatings10020189
- Wang, L., Li, M., Li, X., Liu, J., Mao, Y., Tang, K., "A biomimetic hybrid hydrogel based on the interactions between amino hydroxyapatite and gelatin/gellan gum", Macromolecular Materials and Engineering, Vol. 305, No. 9, (2020), 2000188, 1-9. https://doi.org/10.1002/mame.202000188
- Rodríguez-Vázquez, M., Vega-Ruiz, B., Ramos-Zúñiga, R., Saldaña-Koppel, D. A., Quiñones-Olvera, L. F., "Chitosan and its potential use as a scaffold for tissue engineering in regenerative medicine", BioMed Research International, Vol. 2015, (2015), 821279, 1-16. https://doi.org/10.1155/2015/821279
- Nieto-Suárez, M., López-Quintela, M. A., Lazzari, M., "Preparation and characterization of crosslinked chitosan/gelatin scaffolds by ice segregation induced self-assembly", Carbohydrate Polymers, Vol. 141, (2016), 175-183. https://doi.org/10.1016/j.carbpol.2015.12.064
- Shahin, A., Ramazani S. A., A., Mehraji, S., Eslami, H, "Synthesis and characterization of a chitosan/gelatin transparent film crosslinked with a combination of EDC/NHS for corneal epithelial cell culture scaffold with potential application in cornea implantation", International Journal of Polymeric Materials and Polymeric Biomaterials, Vol. 78, (2020), 1-11. https://doi.org/10.1080/00914037.2020.1865349
- Sarem, M., Moztarzadeh, F., Mozafari, M., "How can genipin assist gelatin/carbohydrate chitosan scaffolds to act as replacements of load-bearing soft tissues?", Carbohydrate Polymers, Vol. 93, No. 2, (2013), 635-643. https://doi.org/10.1016/j.carbpol.2012.11.099
- Manickam, B., Sreedharan, R., Elumalai, M., "'Genipin'-the natural water soluble cross-linking agent and its importance in the modified drug delivery systems: An overview", Current Drug Delivery, Vol. 11, No. 1, (2014), 139-145. https://doi.org/10.2174/15672018113106660059
- Nozari, M., Gholizadeh, M., Zahiri Oghani, F., Tahvildari, K., "Studies on novel chitosan/alginate and chitosan/bentonite flexible films incorporated with ZnO nano particles for accelerating dermal burn healing: In vivo and in vitro evaluation", International Journal of Biological Macromolecules, Vol. 184, (2021), 235-249. https://doi.org/10.1016/j.ijbiomac.2021.06.066
- Yadollahi, M., Gholamali, I., Namazi, H., Aghazadeh, M., "Synthesis and characterization of antibacterial carboxymethyl cellulose/ZnO nanocomposite hydrogels", International Journal of Biological Macromolecules, Vol. 74, (2015), 136-141. https://doi.org/10.1016/j.ijbiomac.2014.11.032
- Murali, S., Kumar, S., Koh, J., Seena, S., Singh, P., Ramalho, A., Sobral, A. J. F. N., "Bio-based chitosan/gelatin/Ag@ZnO bionanocomposites: Synthesis and mechanical and antibacterial properties", Cellulose, Vol. 26, No. 9, (2019), 5347-5361. https://doi.org/10.1007/s10570-019-02457-2
- Mishra, P., Mishra, H., Ekielski, A., Talegaonkar, S., Vaidya, B., "Zinc oxide nanoparticles: A promising nanomaterial for biomedical applications", Drug Discovery Today, Vol. 22, No. 12, (2017), 1825-1834. https://doi.org/10.1016/j.drudis.2017.08.006
- Moshayedi, S., Sarpoolaky, H., Khavandi, A., "Fabrication, swelling behavior, and water absorption kinetics of genipin-crosslinked gelatin–chitosan hydrogels" Polymer Engineering & Science, Vol. 61, No. 12, (2021), 3094-3103. https://doi.org/10.1002/pen.25821
- Li, Y., Sun, S., Gao, P., Zhang, M., Fan, C., Lu, Q., Li, C., Chen, C., Lin, B., Jiang, Y., "A tough chitosan-alginate porous hydrogel prepared by simple foaming method", Journal of Solid State Chemistry, Vol. 294, (2021), 121797, 1-27. https://doi.org/10.1016/j.jssc.2020.121797
- Zhang, Y., Wang, Q. S., Yan, K., Qi, Y., Wang, G. F., Cui, Y. L., "Preparation, characterization, and evaluation of genipin crosslinked chitosan/gelatin three‐dimensional scaffolds for liver tissue engineering applications", Journal of Biomedical Materials Research Part A, Vol. 104, No. 8, (2016), 1863-1870. http://doi.org/ 10.1002/jbm.a.35717
- Sarem, M., Moztarzadeh, F., Mozafari, M., Shastri, V. P., "Optimization strategies on the structural modeling of gelatin/chitosan scaffolds to mimic human miniscus tissue", Materials Science & Engineering: C, Materials for Biological Applications, Vol. 33, No. 8, (2013), 4777-4785. https://doi.org/10.1016/j.msec.2013.07.036
- Rastegar Ramsheh, M., Behnamghader, A., Khanlarkhani, A., "Preparation and characterization of microspheres based on gelatin-sodium alginate-genipin by the formation of polyelectrolyte complex", Journal of Advanced Materials and Technologies (JAMT), Vol. 9, No. 3, (2020a), 63-78. https://doi.org/10.30501/jamt.2021.214580.1070
- Perelshtein, I., Ruderman, E., Perkas, N., Tzanov, T., Beddow, J., Joyce, E., Mason, T. J., Blanes, M., Mollá, K., Patlolla, A., Frenkel, A. I., Gedanken, A., "Chitosan and chitosan–ZnO-based complex nanoparticles: Formation, characterization, and antibacterial activity", Journal of Materials Chemistry: B, Vol. 1, No. 14, (2013), 1968-1976. https://doi.org/10.1039/C3TB00555K
- Tanwar, A., Date, P., Ottoor, D., "ZnO Nps incorporated gelatin grafted polyacrylamide hydrogel nanocomposite for controlled release of ciprofloxacin", Colloid and Interface Science Communications, Vol. 42, (2021), 100413, 1-9. https://doi.org/10.1016/j.colcom.2021.100413
- Amjadi, S., Emaminia, S., Heyat Davudian, S., Pourmohammad, S., Hamishehkar, H., Roufegarinejad, L., "Preparation and characterization of gelatin-based nanocomposite containing chitosan nanofiber and ZnO nanoparticles", Carbohydrate Polymers, Vol. 216, (2019), 376-384. https://doi.org/10.1016/j.carbpol.2019.03.062
- Amira, N., Zain, N., "The effect of ZnO nanoparticles on the physical, mechanical, and antibacterial properties of chitosan/gelatin hydrogel films", Jurnal Teknologi, Vol. 81, (2019), 21-26. https://doi.org/10.11113/jt.v81.12605
- Zhang, M, Qiao, X., Han, W., Jiang, T., Liu, F., Zhao, X., "Alginate-chitosan oligosaccharide-ZnO composite hydrogel for accelerating wound healing", Carbohydrate Polymers, Vol. 266, (2021), 118100, 1-9. https://doi.org/ 10.1016/j.carbpol.2021.11810
- Dinu, M., Dragan, E. S., "Macroporous hydrogels: preparation, properties, and applications", Hydrogels, (2018), 51-85. https://doi.org/10.1007/978-981-10-6077-9_3
- Singhal, R., Gupta, K., "A review: tailor-made hydrogel structures (classifications and synthesis parameters)", Polymer-Plastics Technology and Engineering, Vol. 55, (2015), 1-19. https://doi.org/10.1080/03602559.2015.1050520
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