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هیدروژل تزریقی حساس به دما (کیتوسان/ژلاتین/بتا-گلیسرول فسفات) بهینه‌شده با نانوکامپوزیت پلی‌آنیلین/نانولوله کربن کربوکسیله/ژلاتین حاوی سلول‌های بنیادی در مهندسی بافت قلب

نوع مقاله : مقاله کامل پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، گروه مهندسی علوم زیستی، دانشکده علوم و فنون نوین، دانشگاه تهران، تهران، تهران، ایران

2 دانشیار، گروه مهندسی علوم زیستی، دانشکده علوم و فنون نوین، دانشگاه تهران، تهران، تهران، ایران

3 استادیار دانشکده مهندسی متالوژی و مواد، دانشگاه تهران، تهران، تهران، ایران

4 استاد، مرکز تحقیقات بیمارستان قلب تهران، دانشگاه علوم پزشکی تهران، تهران، تهران، ایران

چکیده

هیدروژل تزریقی که خواص عضله قلب را داشته باشد، چشم‌انداز مهمی در مهندسی بافت قلب است. هدف از این تحقیق، ساخت داربست هیدروژلی قابل تزریق و حساس به دمای کیتوسان/ژلاتین و گلیسرول فسفات است که دارای خواص هدایت‌پذیری و رسانایی باشد تا بتواند با انتقال پالس‌های الکتریکی موجب تسریع در فرایند رشد سلول‌های قلبی و ایجاد بافت قلبی شود. ابتدا نانو­کامپوزیت پلی­ آنیلین/نانولوله کربن چند­دیواره کربوکسیله (PAni/c-MWNT) سنتز شد. برای جلوگیری از واکنش نانوکامپوزیت PAni/c-MWNT با هیدروژل، نانوکامپوزیت با ژلاتین واکنش داده و به شکل PAni/c-MWNT/G درآمد. سپس نانوکامپوزیت PAni/c-MWNT/G در سراسر زمینه کیتوسان ژلاتین به‌منظور ارائه نشانه‌های الکتریکی پراکنده شد. دما و زمان ژل شدن و ویژگی‌های مکانیکی هیدروژل با استفاده از رئومتر اندازه‌گیری شد. طیف FTIR نشان داد برهم‌کنش بین آنیلین و نانولوله کربن موقعیت پیک‌های کنون و بنزن را تغییر داده است. هدایت‌پذیری نانوکامپوزیت در مقایسه با پلیمرهای خالص بیشتر می‌باشد. تصاویر میکروسکوپ الکترونی، توزیع یکنواخت نانوکامپوزیت را در سراسر داربست تأیید کرد. نرخ تخریب داربست رسانا نسبت به داربست خالص کمتر می‌باشد. نتایج آزمایش MTT نشان‌دهنده‌ زیست ­سازگاری هیدروژل با سلول‌ها بود. هیدروژل حاوی سلول‌های بنیادی مزانشیمی به مدت 14 روز کشت داده شد. در این مطالعه برای اولین‌بار، از نانوکامپوزیت پلی ­آنیلین/نانولوله کربن کربوکسیله/ژلاتین (PAni/c-MWNT/G) به‌منظور افزایش هدایت‌پذیری ژل تزریقی کیتوسان/ژلاتین/گلیسرول فسفات برای ساخت داربست رسانا استفاده شده است. این هیدروژل تزریقی رسانا را می‌توان برای بازسازی بافت قلب و همچنین دیگر بافت‌های الکترواکتیو مورد استفاده قرار داد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Injectable Thermosensitive Hydrogel (Chitosan/Gelatin/β-Glycerol Phosphate) Reinforced with Polyaniline/Carboxylated Carbon Nanotube/Gelatin Containing Stem Cells for Cardiac Tissue Engineering

نویسندگان [English]

  • Maryam Kazemi 1
  • Faraz Chogan 1
  • Ali Hossein Rezayan 2
  • Rouhollah Mehdinavaz Aghdam 3
  • Seyed Hossein Ahmadi Tafti 4

1 M. Sc Student., Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Tehran, Iran

2 Associate Professor, Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Tehran, Iran

3 Assistant Professor, School of Metallurgy and Materials Engineering, University of Tehran, Tehran, Tehran, Iran

4 Professor, Tehran Heart Center, Tehran University of Medical Science, Tehran, Tehran, Iran

چکیده [English]

Injectable hydrogels that mimic heart tissues can be considered a promissing perspective towards the future developments of cardiac tissue engineering. This study aims to fabricate an injectable, thermosensitive hydrogel consisting of chitosan/gelatin/glycerol phosphate. Due to their unique electro-conductivity characteristic, hydrogels can provide a suitable environment to accelerate cardiac cell proliferation. Polyaniline/multi-walled carboxylated carbon nanotube (PAni/c-MWNT) was prepared using Sodium Dodecyl Sulfate (SDS) emulsion. To prevent the interaction between the PAni/c-MWNT nanocomposite and hydrogel, the nanocomposite was coated with gelatin to form polyaniline/carboxylated carbon nanotube/gelatin       (PAni/c-MWNT/G). The PAni/c-MWNT/G nanocomposite was then dispersed to provide electrical signals throughout the hydrogel. The gelation time, gel temperature, and mechanical properties of the hydrogel were measured using a rheometer. FTIR spectroscopy results revealed that the interaction between the aniline and      c-MWNT/G could change the position of the quinone and benzene peaks. The conductivity of hydrogel-containing nanocomposite was found to be higher than that of c-MWNT and PAni. Scanning Electron Microscopy (SEM) confirmed the uniform distribution of PAni/c-MWNT/G nanocomposite throughout the hydrogel. The degradation rate of conductive hydrogel is lower than that of pure hydrogel. The MTT assay test showed the biocompatibility of the cell-hydrogel. Finally, Mesenchymal Stem Cells (MSCs) were cultured in the hydrogels for 14 days. Cell adhesion, cell viability, and proliferation were also examined. This study utilized PAni/c-MWNT/G, for the first time, to enhance the electro-conductivity of chitosan/gelatin/glycerol phosphate hydrogel. This conductive thermosensitive injectable hydrogel can be used to regenerate cardiac tissue and other electroactive tissues.

کلیدواژه‌ها [English]

  • Chitosan/Gelatin
  • PAni/c-MWNT
  • Thermosensitive Conductive
  • Hydrogels
  • Cardiac Tissue Engineering
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مواد و فناوری‌های پیشرفته
دوره 11، شماره 2
شهریور 1401
صفحه 71-87
فایل ها
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  • تاریخ دریافت: 22 دی 1400
  • تاریخ بازنگری: 04 اردیبهشت 1401
  • تاریخ پذیرش: 09 بهمن 1401
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