نوع مقاله : مقاله کامل پژوهشی
نویسندگان
1 دانشجوی دکتری، پژوهشکده فناوری نانو و مواد پیشرفته، پژوهشگاه مواد و انرژی، کرج، ایران
2 دانشیار، پژوهشکده فناوری نانو و مواد پیشرفته، پژوهشگاه مواد و انرژی، کرج، ایران
3 استاد، پژوهشکده فناوری نانو و مواد پیشرفته، پژوهشگاه مواد و انرژی، کرج، ایران
چکیده
آلفا-تری‌کلسیم فسفات، به‌عنوان جزء پودری در فرآیند تهیه سیمان‌های کلسیم فسفاتی جهت بازسازی بافت سخت استفاده می‌شود. در این تحقیق، پودر مذکور از مواد اولیه نیترات کلسیم و دی‌آمونیوم هیدروژن فسفات به روش رسوب‌دهی شیمیایی سنتز شد. پودر حاصل در 1250 درجه سلسیوس تحت عملیات حرارتی قرار گرفت و در دمای محیط به‌سرعت سرد شد. نتایج آزمون پراش اشعه ایکس (XRD) و طیف‌سنجی فروسرخ تبدیل فوریه (FTIR) پودر سنتز‌شده، تشکیل فاز آلفا-تری‌کلسیم فسفات بلوری و وجود گروه‌های شیمیایی P-O را تأیید کرد. سیمان تک‌جزئی با استفاده از پودر آلفا-تری‌کلسیم فسفات و فاز مایع حاوی 5/2 درصد دی‌سدیم هیدروژن فسفات تهیه شد. سیمان حاصل با زمان گیرش اولیه 1 17 دقیقه، دارای استحکام فشاری 2 21 مگاپاسکال بود. آزمایش‌های XRD و FTIR تشکیل مقدار زیادی هیدروکسی‌آپاتیت با کمبود کلسیم را به‌عنوان محصول سیمان تأیید کرد. طبق یافته‌ها، گیرش سیمان از طریق آب‌کافت پودرهای آلفا-تری‌کلسیم فسفات و تشکیل نانو‌فلس‌های هیدروکسی‌آپاتیت با کمبود کلسیم به طول تقریبی 500 نانومتر اتفاق افتاده است. آزمایش زیست‌فعالی، تشکیل هیدروکسی‌آپاتیت در سطح خارجی سیمان را طی 14 روز غوطه‌وری در مایع شبیه‌سازی‌شده بدن، نشان داد.
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
Synthesis and Characterization of Alpha-Tricalcium Phosphate and Its Single-Component Cement
نویسندگان [English]
- Rahim Jahandideh 1
- Aliasghar Behnamghader 2
- Saeed Hesaraki 3
1 Ph. D. Candidate, Department of Nanotechnology and Advanced Materials, Materials and Energy Research Center, Karaj, Iran
2 Associate Professor, Department of Nanotechnology and Advanced Materials, Materials and Energy Research Center, Karaj, Iran
3 Professor, Department of Nanotechnology and Advanced Materials, Materials and Energy Research Center, Karaj, Iran
چکیده [English]
Alpha-tricalcium phosphate can be used as a powder component in the preparation process of calcium phosphate cements in hard tissue applications. In this study, the mentioned powder was synthesized through chemical precipitation method using calcium nitrate and diammonium hydrogen phosphate as the raw materials. The resulting powder was heat-treated at 1250 °C and quenched at the ambient temperature. The results of X-Ray Diffraction (XRD) analysis and Fourier Transform Infrared (FTIR) spectroscopy confirmed the formation of crystalline Alpha-tricalcium phosphate phase and presence of P-O chemical groups, respectively. The Single-component cement was prepared using Alpha-tricalcium phosphate powder with the liquid phase containing 2.5 % disodium hydrogen phosphate. The resulting cement sample had an initial setting time of 17 1 minute and the compressive strength of 21 2 MPa. The XRD and FTIR experiments revealed the formation of a great amount of calcium deficient hydroxyapatite as the resulting cement product. According to the findings, the cement setting occurred through the hydrolysis of Alpha-tricalcium phosphate powders and the formation of calcium deficient hydroxyapatite nanoflakes of approximately 500 nm in length. Finally, the cement acellular bioactivity experiment confirmed that the hydroxyapatite was formed on the outer surface of the cement during 14 days of immersion in the simulated body fluid.
کلیدواژه‌ها [English]
- Alpha-Tricalcium Phosphate
- Synthesis
- Cement
- Calcium Deficiency Hydroxyapatite
- Almirall, A., Larrecq, G., Delgado, J. A., Martinez, S., Planell, J. A., Ginebra M. P., "Fabrication of low temperature macroporous hydroxyapatite scaffolds by foaming and hydrolysis of an α-TCP paste", Biomaterials, 25, No. 17, (2004), 3671-3680. https://doi.org/10.1016/j.biomaterials.2003.10.066
- Brown, W. E., Chow, L.C., "Dental resptorative cement pastes", U.S. Patent 4518430 A, (1985). https://www.freepatentsonline.com/4518430.html
- Hideki, M., Takafumi, K., "The hydration of α-tricalcium phosphate", Journal of the Ceramic Association, Vol. 84, No. 968, (1976), 209-213. https://doi.org/10.2109/jcersj1950.84.968_209
- Carrodeguas, R. G., De Aza, S., "α-Tricalcium phosphate: Synthesis, properties and biomedical applications", Acta Biomaterialia, Vol. 7, No. 10, (2011), 3536-3546. https://doi.org/10.1016/j.actbio.2011.06.019
- Dorozhkin, S. V., "Self-setting calcium orthophosphate formulations", Journal of Functional Biomaterials, 4, No. 4, (2013), 209-311. https://doi.org/10.3390/jfb4040209
- Valle, S., Mino, N., Munoz, F., Gonzalez, A., Planell, J. A., Ginebra, M. P., "In vivo evaluation of an injectable macroporous calcium phosphate Cement", Journal of Materials Science: Materials in Medicine, 18, (2007), 353-361. https://doi.org/10.1007/s10856-006-0700-y
- LeGeros, R. Z., Parsons, J. R., Daculsi,, Driessens, F., Lee, D., Liu, S. T., Metsger, S., Peterson, D., Walker, M., "Significance of the porosity and physical chemistry of calcium phosphate ceramics", Bioceramics: Material Characteristics Versus in Vivo Behavior, Vol. 523, No. 1, (1988), 268-271. https://doi.org/10.1111/j.1749-6632.1988.tb38519.x
- Shimazaki, K., Mooney, V., "Comparative study of porous hydroxyapatite and tricalcium phosphate as bone substitute", Journal of Orthopaedic Research, 3, No. 3, (1985), 301-310. https://doi.org/10.1002/jor.1100030306
- Montufar, E. B., Traykova, T., Gil, C., Harr, I., Almirall, A., Aguirre, A., Engel, E., Planell, J. A., Ginebra. M. P., "Foamed surfactant solution as a template for self-setting injectable hydroxyapatite scaffolds for bone regeneration", Acta Biomaterialia, Vol. 6, No. 3, (2010), 876-885. https://doi.org/10.1016/j.actbio.2009.10.018
- Asadi Tabrizi, R., Zamanian. A., Hesaraki. S., "Effects of time, temperature and precursor on solid state synthesis of α-TCP", Advanced Ceramics Progress, Vol. 1, No. 1, (2015) 36-39. https://www.sid.ir/en/journal/ViewPaper.aspx?id=458574
- American Society for Testing and Material (ASTM), "ASTM C266-89 A: Standard test method for time of setting of hydraulic-cement paste by gillmore needles", West Conshohocken, PA, (2004). Available at: https://www.astm.org/DATABASE.CART/HISTORICAL/C266-04.htm
- Kokubo, T., Takadama, H., "How useful is SBF in predicting in vivo bone bioactivity", Biomaterials, 27, No. 15, (2006), 2907-2915. https://doi.org/10.1016/j.biomaterials.2006.01.017
- Chen, F., Song, Z., Liu, Ch., "Fast setting and anti-washout injectable calcium–magnesium phosphate cement for minimally invasive treatment of bone defects", Journal of Materials Chemistry B, Vol. 3, No. 47, (2015), 9173-9181. https://doi.org/10.1039/c5tb01453k
- Xie, Y., Liu, J., Cai, Sh., Bao, X., Li, Q., Xu, G., "Setting characteristics and high compressive strength of an anti-washout, injectable calcium phosphate cement combined with thermosensitive hydrogel", Materials, Vol. 13, No. 24, (2020), 5779. https://doi.org/10.3390/ma13245779
- Ginebra, M. P., Fernandez, E., De Maeyer, E. A. P, Verbeeck, R. M. H., Boltong, M. G., Ginebra, J., Driessens, F. C. M., Planell, J. A., "Setting reaction and hardening of an apatitic calcium phosphate cement", Journal of Dental Research, 76, No. 4, (1997), 905-912. https://doi.org/10.1177/00220345970760041201
- Ginebra, M. P., Fernandez, E., Driessens, F. C. M., Planell, J. A., "Modeling of the hydrolysis of α-tricalcium phosphate", Journal of the American Ceramic Society, 82, No. 10, (1999), 2808-2812. https://doi.org/10.1111/j.1151-2916.1999.tb02160.x
- Sampath Kumar, T. S., "Physical and chemical characterization of biomaterials”, Characterization of Biomaterials, Bandyopadhyay, A., Bose, S., (ed.), Academic Press, (2013), 11-47. https://doi.org/10.1016/B978-0-12-415800-9.00002-4
- Berzina-Cimdina, L., Borodajenko, N., "Research of calcium phosphates using fourier transform infrared spectroscopy", Theophile, T., Infrared Spectroscopy - Materials Science, Engineering and Technology, IntechOpen, (2012), 123-148. https://doi.org/5772/36942
- Ramanan, S. R., Venkatesh, R., "A study of hydroxyapatite fibers prepared via sol-gel route", Materials Letters, Vol. 58, No. 26, (2004), 3320-3323. https://doi.org/10.1016/j.matlet.2004.06.030
- Montel, G., Bonel, G., Heughebaert, J. C., Trombe, J. C., Rey, C., "New concepts in the composition, crystallization and growth of the mineral component of calcified tissues", Journal of Crystal Growth, Vol. 53, No. 1, (1981), 74-99. https://doi.org/10.1016/0022-0248(81)90057-9
- Gil, F. J., Padro’s, A., Manero, J. M., Aparicio, C., Nilsson, M., Planell, J. A., "Growth of bioactive surfaces on titanium and its alloys for orthopaedic and dental implants", Materials Science and Engineering: C, 22, No. 1, (2002), 53-60. https://doi.org/10.1016/S0928-4931(01)00389-7
- Feng, B., Guolin, M., Yuan, Y., Changshen, L., Zhen, W., Jian, L., "Role of macropore size in the mechanical properties and in vitro degradation of porous calcium phosphate cements", Materials Letters, Vol 64, No. 18, (2010), 2028-2031. https://doi.org/10.1016/j.matlet.2010.06.008
- Lu, J., Descamps, M., Dejou, J., Koubi, G., Hardouin, P., Lematre, J., Proust, J. P., "The biodegradation mechanism of calcium phosphate biomaterials in bone", Journal of Biomedical Materials Research, Vol. 63, 4, (2002), 408-412 https://doi.org/10.1002/jbm.10259
)