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تأثیر افزودن عامل شبکه‌ای‌کننده گلیسیدوکسی پروپیل تری‌متوکسی سیلان در خواص داربست‌های ژلاتین/کیتوسان تهیه‌شده به روش خشکاندن انجمادی و معرفی شرایط بهینه شبکه‌ای‌ کردن داربست‌های کیتوسان/ژلاتین برای کاربردهای مهندسی بافت استخوان

نوع مقاله : مقاله کامل پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه بیومواد، پژوهشکده فناوری نانو و مواد پیشرفته، پژوهشگاه مواد و انرژی، کرج، ایران

2 استاد، گروه بیومواد، پژوهشکده فناوری نانو و مواد پیشرفته، پژوهشگاه مواد و انرژی، کرج، ایران

چکیده

گلیسیدوکسی پروپیل تری‌متوکسی سیلان (GPTMS)، افزون بر بهبود عملکرد زیست‌فعالی و سلولی داربست‌ها، به افزایش پایداری و استحکام هیدروژل‌ها در کاربردهای استخوانی منجر می‌شود. درصد عامل شبکه‌ای‌کننده افزوده‌شده یکی از عوامل مؤثر در خواص داربست است. به همین دلیل، در این مطالعه، داربست‌های کیتوسان و ژلاتین با درصدهای وزنی گوناگون GPTMS به روش خشکاندن انجمادی آماده و ارزیابی شدند. نتایج میکروسکوپ الکترونی روبشی (SEM) نشانگر دستیابی به داربست‌های متخلخل است که اندازه تخلخل‌ها، با افزایش مقدار عامل شبکه‌ای‌کننده، به حدود 290 میکرون رسیدند. نتایج طیف‌سنجی مادون قرمز تبدیل فوریه نشان داد که پلیمر‌ها و فرایند شبکه‌ای‌شدن با تشکیل گروه‌های سیلانه برهم‌کنش دارند. همچنین، زاویه تماس با افزایش مقدار عامل شبکه‌ای‌کننده کاهش یافت و نمونه دارای 75 درصد وزنی GPTMS دارای زاویه تماس مطلوب 5/3 ± 7/60 درجه بود و درصد تورم و تخریب کاهش یافت. بااین‌حال، خواص مکانیکی با افزودن GPTMS تا 267 ± 1590 کیلوپاسکال افزایش یافت. همچنین، GPTMS به افزایش خواص زیست‌فعالی و رسوب لایه هیدروکسی آپاتیت منجر شد که الگوی تفرق پرتو ایکس نیز این ادعا را تأیید می‌کند. به نظر می‌رسد که داربست ژلاتین – کیتوسان، با 75 درصد وزنی GPTMS، بهترین نمونه برای استفاده در مهندسی بافت استخوان است.

کلیدواژه‌ها

عنوان مقاله [English]

The Effect of Adding Different Percentages of γ-Glycidoxypropyltrimethoxysilane Cross-linker on the Properties of Freeze-dried Gelatin/Chitosan Scaffolds upon Introducing an Optimum Condition to Cross-link the Chitosan/Gelatin Scaffolds in Bone Tissue Engineering

نویسندگان [English]

  • Fatemeh Banafatizadeh 1
  • Ali Zamanian 2

1 Ph. D. Candidate, Biomaterials Research Group, Department of Nanotechnology and Advanced Materials, Materials and Energy Research Center, Karaj, Iran

2 Professor, Biomaterials Research Group, Department of Nanotechnology and Advanced Materials, Materials and Energy Research Center, Karaj, Iran

چکیده [English]

Use of γ-glycidoxypropyltrimethoxysilane (GPTMS) enhances the stability and strength of hydrogels and improves the bioactivity and cellular performance of the scaffolds in bone applications. Given that one of the leading factors that affects the properties of the scaffold is the percentage of the cross-linking agent, chitosan and gelatin scaffolds with different percentages of GPTMS were prepared and evaluated through freeze-drying method. The results from Scanning Electron Microscope (SEM) confirmed the achievement of porous scaffolds with open pores and upon increasing the amount of cross-linking agent, the size of the pores reached approximately 290 microns. The results of the infrared spectrum showed the interaction among the polymers and process of cross-linking with the formation of silane groups. In this study, upon increasing the amount of the transverse bonding agent, the contact angle decreased, the optimal contact angle of the sample with 75 (weight percent) GPTMS reached 60.7 ± 3.5 degrees, and the percentages of both swelling and degradation ultimately decreased. It should be noted that the mechanical properties were improved by adding GPTMS up to 1590 ± 267 kPa. According to the findings of this study, application of GPTMS led to an enhancement in the bioactivity properties and deposition of the hydroxyapatite layer, and the X-ray diffraction pattern confirmed this claim. The obtained results support the hypothesis that gelatin-chitosan scaffolds with 75 (wt %) GPTMS seem to be the best samples for use in bone tissue engineering.

کلیدواژه‌ها [English]

  • Gelatin
  • Chitosan
  • Cross-linker
  • Bone Tissue Engineering
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مواد و فناوری‌های پیشرفته
دوره 12، شماره 2
شهریور 1402
صفحه 39-54
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  • تاریخ دریافت: 14 تیر 1401
  • تاریخ بازنگری: 19 شهریور 1401
  • تاریخ پذیرش: 23 شهریور 1401
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