Skin, as the largest body organ, performs several functions namely creating a protective barrier against microorganisms and regulating body temperature. For this reason, maintenance of skin integrity and health is of high importance. This tissue can repair itself in limited damages; however, under some conditions such as severe burns, extensive and deep damage, and chronic wounds, skin loses its ability to completely repair itself. For this reason, the products that help repair the wound are needed. Such skin products are prepared through skin tissue engineering. In this respect, extensive research has been done, and natural and synthetic materials with different potentials were used to prepare wound dressings. One of these materials is the eggshell membrane which is a fibrous structure between the inner layer of the mineral shell and the egg white. This membrane has attracted a number of researchers’ attention due to its porous structure, biocompatibility, availability, non-contamination, containing natural proteins and compounds similar to the extracellular matrix, and low cost. It has many applications in medicine, among which is preparation of wound dressings and wound healing products with promising results. In this study, eggshell membrane is introduced as a natural biomaterial. Further, its application in wound healing and preparation of wound dressings are reviewed.