مواد و فناوری‌های پیشرفته

مواد و فناوری‌های پیشرفته

ساخت و مشخصه‌یابی فیلم زانتان حاوی عصاره اسپیرولینا با قابلیت رهایش پیوسته به‌منظور کاربرد در ترمیم زخم

نوع مقاله : مقاله کامل پژوهشی

نویسندگان
افروز قاسم زاده 1
رعنا ایمانی 2
طهورا ابراهیمی 3
1 دانشجوی کارشناسی، دانشکده مهندسی پزشکی، دانشگاه صنعتی امیرکبیر (پلی‌تکنیک تهران)، تهران، ایران
2 استادیار، دانشکده مهندسی پزشکی، دانشگاه صنعتی امیرکبیر (پلی‌تکنیک تهران)، تهران، ایران
3 دانشجوی دکتری، دانشکده مهندسی پزشکی، دانشگاه صنعتی امیرکبیر (پلی‌تکنیک تهران)، تهران، ایران
10.30501/jamt.2023.364845.1272
چکیده
پوست مانعی برای ورود آلودگی‌ها به بدن است. بنابراین، در صورت ایجاد زخم، لازم است به ترمیم سریع آن پرداخته شود. امروزه، متداول‌ترین روش برای درمان زخم استفاده از زخم‌پوش‌های مهندسی‌شده ‌است. این زخم‌پوش‌ها باید، با ارائه هدفمند و پیوسته‌ ترکیبات مؤثر، باعث تسریع فرایند بهبود شوند. در پژوهش پیش‌رو، زخم‌پوش فیلمی برمبنای پلیمر زیستی زانتان حاوی نانوحامل نیوزومی برای رهایش موضعی عصاره جلبک اسپیرولینا تهیه شد. نانوحامل‌های نیوزومی حاوی عصاره اسپیرولینا با روش هیدراسیون فیلم نازک تهیه شدند. سپس، زخم‌پوش فیلمی، با ترکیب‌ کردن یکنواخت زانتان، ماده نرم‌کننده گلیسیرین و نیوزوم‌های حاوی اسپیرولینا به دست آمد. برای بررسی ویژگی زخم‌پوش تهیه‌شده، آزمون‌های ارزیابی متفاوتی انجام شد. با توجه به بررسی‌های میکروسکوپ ‌الکترونی ‌روبشی، ریخت‌شناسی نیوزوم‌های کروی با میانگین اندازه حدود 200 نانومتر بود و درصد بارگذاری دارو درون نیوزوم‌ها 72 درصد به دست آمد. همچنین، 25 درصد دارو از ساختار فیلم حاوی نیوزوم طی 20 روز رهایش می‌یابد. به‌علاوه، زخم‌پوش حاوی نیوزوم طی 20 روز کاهش وزن 35 درصد را نشان می‌دهد. با انجام آزمون سمیت سلولی طی 24 ساعت مشخص شد که تا غلظت 200 میکروگرم/میلی‌لیتر از عصاره اسپیرولینا زنده‌مانی سلول‌ها در مقایسه با نمونه کنترل بالای 80 درصد است. درمجموع، نتایج این مطالعه نشان می‌دهد که حامل نیوزومی در ساختار فیلم زانتان می‌تواند به‌عنوان سامانه دارورسان موضعی مناسب برای درمان زخم استفاده شود.
کلیدواژه‌ها
زخم‌پوش
صمغ زانتان
عصاره اسپیرولینا
رهایش کنترل‌شده
نیوزوم

موضوعات

عنوان مقاله English

Fabrication and Characterization of Xanthan Film Containing Spirulina Extract with Continues Release Capability for Use in Wound Healing

نویسندگان English

Afrouz Ghasemzadeh 1
Rana Imani 2
Tahura Ebrahimi 3
1 BSc Student, Biomedical Engineering Department, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran.
2 Assistant Professor, Biomedical Engineering Department, Amirkabir University of Technology (Tehran Polytechnic), Tehran. Tehran, Iran.
3 PhD Candidate, Biomedical Engineering Department, Amirkabir University of Technology (Tehran Polytechnic), Tehran. Iran
چکیده English

Today, the most common method for wound treatment is to use engineered wound dressings. In the present study, a film wound dressing based on xanthan biopolymer, containing niosomal nanocarrier, was prepared for the local release of spirulina algae extract. Niosomal nanocarriers containing the spirulina extract were prepared using thin film hydration method. Then, a film wound dressing was produced by uniformly combining xanthan, glycerol emollient, and niosomes containing spirulina. According to scanning electron microscopy, the morphology of the niosomes were spherical with an average size of about 200 nm, and the percentage of drug loading within the niosomes was 72%. Moreover, 25% of the drug was released from the structure of the film containing niosomes within 20 days. Additionally, the wound dressing containing niosomes exhibited a 35% weight loss within 20 days. Through cytotoxicity testing conducted within 24 hours, it was revealed that up to a concentration of 200 μg/ml of the spirulina drug, cell viability remained higher than 80% compared to the control sample. In summary, the results of this study indicate that the niosomal carrier within the xanthan film structure could serve as an effective local drug delivery system for wound treatment.

کلیدواژه‌ها English

Wound Dressing
Xanthan Gum
Spirulina Extract
Controlled Release
Niosome
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مواد و فناوری‌های پیشرفته
دوره 12، شماره 4
زمستان 1402
صفحه 1-14

  • تاریخ دریافت 20 فروردین 1402
  • تاریخ بازنگری 24 تیر 1402
  • تاریخ پذیرش 05 دی 1402