نوع مقاله : مقاله کامل پژوهشی
نویسندگان
1 دانشجوی کارشناسی‌ارشد، دانشکده مهندسی شیمی، واحد علوم تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران.
2 دانشیار، گروه شیمی، دانشکده علوم پایه، واحد ورامین- پیشوا، دانشگاه آزاد اسلامی، ورامین، ایران
چکیده
اصلاح سطح گرافن اکسید (GO) با مولکولهای زیستی می‌تواند بر محدودیت‌های آن از نظر زیست سازگاری، ورود به درون سلول و اثربخشی در دارورسانی غلبه کند و طیف وسیعی از کاربردهای زیست پزشکی را ممکن سازد. در این پژوهش، بیوکامپوزیت گرافن اکسید مغناطیسی عامل دارشده با آمینو اسید L-گلوتامین (L-Gln/MGO) تهیه شد و به عنوان نانوحاملی مناسب با ظرفیت بارگیری دارو بالا و خواص آزادسازی عالی برای 5-فلوئورواوراسیل (5FU)، یک داروی ضد سرطان به کار برده شد. pH بهینه برای بیشترین میزان جذب دارو در 20 درجة سلسیوس، 4 بود. به دلیل ماهیت گرماده فرایند جذب، با افزایش دما ظرفیت جذب کاهش یافت. برخی مدل‌های شناخته شده، از جمله شبه مرتبة اول، شبه مرتبة دوم و انتشار درونذره‌ای، برای بررسی سینتیک جذب به کار رفتند. علاوه بر این، مدل‌های لانگمویر، فروندلیچ و ردلیچ-پیترسون برای مطالعة هم دماهای جذب مورد استفاده قرار گرفتند. نتایج حاصل نشان داد که فرایند جذب پیرو مدل های هم دمای لانگمویر و سینتیک شبه مرتبة دوم است. حدود 26 درصد از 5FU در مایع شبیه سازی شدة معده (pH 2/1) در 37 درجة سلسیوس، در 30 دقیقه اول رها شد، در صورتی که 32 درصد دیگر در مایع شبیه سازی شدة روده (pH 4/7)، در طول 30 ساعت بعد آزاد شد. نتایج به‌دست‌آمده می تواند برای طراحی یک سامانة بارگذاری کنترل شده و دارورسانی هدفمند 5FU مفید باشد.
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
Fabrication and Characterization of L-Glutamine-Functionalized Magnetic Graphene Oxide as a Novel Bio-Platform for Controlled Release of 5-Fluorouracil
نویسندگان [English]
- Mohammad Sharifpour 1
- Mahsasadat Miralinaghi 2
1 MSc Student, Department of Chemical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2 Associate Professor, Department of Chemistry, Faculty of Science, Varamin - Pishva Branch, Islamic Azad University, Varamin, Iran.
چکیده [English]
Surface modification with biomolecules can overcome Graphene Oxide (GO) restrictions in biocompatibility, cellular internalization, and drug delivery effectiveness, hence suitable for a wide range of biomedical applications. In this study, a biocomposite (L-glutamine-functionalized magnetic graphene oxide (L-Gln/MGO)) was prepared and used as a suitable nanoscale carrier with high drug loading capacity and excellent release properties for 5-fuorouracil (5FU), an anticancer drug. The optimum pH for maximum drug adsorption was determined as 4 at 293 K and as the temperature increased, the adsorption capacity decreased due to the exothermic nature of the adsorption process. Some well-known models, including pseudo-first-order, pseudo-second-order, and Intraparticular Diffusion (IPD), were applied to examine the kinetics of adsorption. Additionally, the Langmuir, Freundlich, and Redlich-Peterson models were used to investigate the adsorption isotherms. The obtained results showed that the adsorption process adhered to the Langmuir isotherm and pseudo-second-order kinetic models. Nearly 26% of 5FU was released in the simulated stomach fluid at the pH of 1.2 and temperature of 37 °C in the first 30 minutes while 32% of which was released in the simulated intestinal fluid at the pH of 7.4 during the next 30 hours. The obtained results might be helpful for designing a controllable loading and targeted 5FU drug delivery system.
کلیدواژه‌ها [English]
- Nanocomposite
- Adsorption
- Controlled Release
- Targeted Drug Delivery
- Abdi, Z., Malek Khachatourian, A., & Nemati, A. (2022). Studying the Effect of Calcination on the Optical and Magnetic Properties of NiFe2O4@ZnO:Ti Nanoparticles. Advanced Ceramics Progress, 8(3), 1–7. https://doi.org/10.30501/ACP.2022.363261.1101
- Asemaneh, H. R., Rajabi, L., Dabirian, F., Rostami, N., Derakhshan, A. A., & Davarnejad, R. (2020). Functionalized Graphene Oxide/Polyacrylonitrile Nanofibrous Composite: Pb2+ and Cd2+ Cations Adsorption. International Journal of Engineering, 33(6), 1048–1053. https://doi.org/10.5829/IJE.2020.33.06C.01
- Ashuri, A., Miralinaghi, M., & Moniri, E. (2022). Evaluation of folic acid-conjugated chitosan grafted Fe3O4/graphene oxide as a pH- and magnetic field-responsive system for adsorption and controlled release of gemcitabine. Korean Journal of Chemical Engineering, 39(7), 1880–1890. https://doi.org/10.1007/S11814-022-1104-5
- Azizi, M., & Kalantar, M. (2020). Evaluation of Microstructural and Antibacterial Properties of Graphene Oxide Synthesized by Green Method. Journal of Advanced Materials and Technologies, 9(3), 51–61. https://doi.org/10.30501/JAMT.2020.221997.1075
- Daneshmoghanlou, E., Miralinaghi, M., Moniri, E., & Sadjady, S. K. (2022). Fabrication of a pH-Responsive Magnetic Nanocarrier Based on Carboxymethyl Cellulose-Aminated Graphene Oxide for Loading and In-Vitro Release of Curcumin. Journal of Polymers and the Environment, 30(9), 3718–3736. https://doi.org/10.1007/S10924-022-02467-5
- Hummers Jr, W. S., & Offeman, R. E. (1958). Preparation of Graphitic Oxide. Journal of the American Chemical Society, 80(6), 1339–1339. https://doi.org/10.1021/ja01539a017
- Itoo, A. M., Vemula, S. L., Gupta, M. T., Giram, M. V., Kumar, S. A., Ghosh, B., & Biswas, S. (2022). Multifunctional graphene oxide nanoparticles for drug delivery in cancer. Journal of Controlled Release, 350, 26–59. https://doi.org/10.1016/J.JCONREL.2022.08.011
- Liu, J., Cui, L., & Losic, D. (2013). Graphene and graphene oxide as new nanocarriers for drug delivery applications. Acta Biomaterialia, 9(12), 9243–9257. https://doi.org/10.1016/J.ACTBIO.2013.08.016
- Makvandi, P., Ghomi, M., Ashrafizadeh, M., Tafazoli, A., Agarwal, T., Delfi, M., Akhtari, J., Zare, E. N., Padil, V. V. T., Zarrabi, A., Pourreza, N., Miltyk, W., & Maiti, T. K. (2020). A review on advances in graphene-derivative/polysaccharide bionanocomposites: Therapeutics, pharmacogenomics and toxicity. Carbohydrate Polymers, 250, 116952. https://doi.org/10.1016/J.CARBPOL.2020.116952
- Milas, L., Mason, K. A., Hunter, N., Li, C., & Wallace, S. (2003). Poly(L-glutamic acid)-paclitaxel conjugate is a potent enhancer of tumor radiocurability. International Journal of Radiation Oncology *Biology* Physics, 55(3), 707–712. https://doi.org/10.1016/S0360-3016(02)04153-6
- Orsu, P., & Koyyada, A. (2020). Recent progresses and challenges in graphene based nano materials for advanced therapeutical applications: a comprehensive review. Materials Today Communications, 22, 100823. https://doi.org/10.1016/J.MTCOMM.2019.100823
- Pooresmaeil, M., & Namazi, H. (2023). pH-sensitive carboxymethyl starch-gelatin coated COF/5-Fu for colon cancer therapy. Industrial Crops and Products, 202, 117102. https://doi.org/10.1016/J.INDCROP.2023.117102
- Rao, Z., Ge, H., Liu, L., Zhu, C., Min, L., Liu, M., Fan, L., & Li, D. (2018). Carboxymethyl cellulose modified graphene oxide as pH-sensitive drug delivery system. International Journal of Biological Macromolecules, 107(PartA), 1184–1192. https://doi.org/10.1016/J.IJBIOMAC.2017.09.096
- Rezakhani, D., Jafari, A. H., & Hajabassi, M. A. (2022). Modified Concrete for Impeding Chloride Diffusion from Sea Water in the Marine Environment. Journal of Renewable Energy and Environment, 9(3), 17–31. https://doi.org/10.30501/JREE.2022.293613.1227
- Rokni, S. E., Haji Seyed Mohammad Shirazi, R., Miralinaghi, M., & Moniri, E. (2020). Efficient adsorption of anionic dyes onto magnetic graphene oxide coated with polyethylenimine: Kinetic, isotherm, and thermodynamic studies. Research on Chemical Intermediates, 46(4), 2247–2274. https://doi.org/10.1007/s11164-020-04090-2
- Shah, S., Famta, P., Bagasariya, D., Charankumar, K., Amulya, E., Khatri, D. K., Singh Raghuvanshi, R., Bala Singh, S., & Srivastava, S. (2022). Nanotechnology based drug delivery systems: Does shape really matter?. International Journal of Pharmaceutics, 625, 122101. https://doi.org/10.1016/J.IJPHARM.2022.122101
- Siegel, R. L., Miller, K. D., Wagle, N. S., & Jemal, A. (2023). Cancer statistics, 2023. Ca Cancer J Clin, 73(1), 17-48. https://doi.org/10.3322/CAAC.21763
- Yadav, S., Asthana, A., Singh, A. K., Chakraborty, R., Vidya, S. S., Susan, M. A. B. H., & Carabineiro, S. A. C. (2021). Adsorption of cationic dyes, drugs and metal from aqueous solutions using a polymer composite of magnetic/β-cyclodextrin/activated charcoal/Na alginate: Isotherm, kinetics and regeneration studies. Journal of Hazardous Materials, 409, 124840. https://doi.org/10.1016/J.JHAZMAT.2020.124840
)