تأثیر درجه اکسیداسیون آلژینات بر خواص جوهر زیستیِ خودبخود ژل‌شوندهِ آلژینات اکسیدشده-ژلاتین در فرایند چاپ زیستی سه‌بعدی

سنایی, خدیجه; زمانیان, علی; مشایخان, شهره; رمضانی فرزین, طیبه

doi:10.30501/jamt.2023.395058.1277

تأثیر درجه اکسیداسیون آلژینات بر خواص جوهر زیستیِ خودبخود ژل‌شوندهِ آلژینات اکسیدشده-ژلاتین در فرایند چاپ زیستی سه‌بعدی

نوع مقاله : مقاله کامل پژوهشی

نویسندگان

خدیجه سنایی ¹

علی زمانیان ²

شهره مشایخان ³

طیبه رمضانی فرزین ⁴

¹ دانشجوی دکتری، پژوهشکده فناوری نانو و مواد پیشرفته، پژوهشگاه مواد و انرژی، کرج، ایران

² استاد، پژوهشکده فناوری نانو و مواد پیشرفته، پژوهشگاه مواد و انرژی، کرج، ایران

³ دانشیار، دانشکده شیمی و نفت، دانشگاه شریف، تهران، ایران

⁴ دکتری، دانشکده علوم زیستی، دانشگاه خوارزمی، تهران، ایران

10.30501/jamt.2023.395058.1277

چکیده

استفاده از آلژینات اکسیدشده یا آلژینات دیآلدئیدی، بهدلیل حذف یا تقلیل عاملهای شبکهساز شیمیایی، در حوزه‌های مختلف به‌ویژه چاپ زیستیِ سه‌بعدی موردتوجه قرار گرفته است. در این پژوهش آلژینات بهمیزان 5، 5/7 و 10 درصد اکسید شد و ترکیب شیمیایی و وزن مولکولی آلژینات و اکسید آن، قبل و بعد از فرایند اصلاح شیمیایی اکسیداسیون بهترتیب با طیف‌سنجی تبدیل فوریه مادون‌قرمز (FTIR)، آزمون رزونانس مغناطیسی هسته (NMR) و آزمون کروماتوگرافی نفوذ ژل (GPC) بررسی و نیز خواص رئولوژی مطالعه شد. سپس جوهر زیستی با استفاده از آلژینات اکسیدشده (با درجه اکسیداسیونهای مختلف 5، 5/7 و 10 درصد) با غلظت 4 درصد (وزنی به حجمی) و ژلاتین 6 درصد (وزنی به حجمی)، تهیه شد و هیدروژلها تحت یک فرایند دومرحلهای، اتصال عرضی (cross link) شدند. در ادامه، خواص جوهر زیستی از جمله سیالیت، چاپپذیری و ترشوندگی بررسی شد. نتایج نشان داد که اکسیداسیون آلژینات موجب کاهش جرم مولکولی و کاهش خواص رئولوژی آلژینات میشود. بااین‌وجود، بررسی خواص جوهر زیستی (آلژینات اکسیدشده-ژلاتین) نشان داد که در نمونه با درجه اکسیداسیون 5 درصد، خواص چاپپذیری، شبکهای شدن و ترشوندگی در محدوده مناسب قرار دارد و میتوان از آن به‌عنوان جوهر زیستی ایدهآل در چاپ زیستی استفاده کرد.

کلیدواژه‌ها

چاپ زیستی سه بعدی

جوهر زیستی

آلژینات اکسید شده

موضوعات

بیوپلیمرها (کیتوسان، ...)

عنوان مقاله English

Effect of Oxidation Degree (OD) of Oxidized-Alginate on the Properties of Self-Vrosslinkable Oxidized Alginate-Gelatin Bioinks in 3D Bioprinting Process

نویسندگان English

khadijeh Sanaei ¹

Ali Zamanian ²

Shohreh Mashayekhan ³

Tayebeh Ramezani Farzin ⁴

¹ Ph. D. Candidate, Department of Nanotechnology and Advanced Materials, Materials and Energy Research Center, Karaj, Iran.

² Professor, Department of Nanotechnology and Advanced Materials, Materials and Energy Research Center, Karaj, Iran.

³ Associate Professor, Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran, Iran.

⁴ Ph.D, Faculty of biological sciences, Kharazmi University, Tehran, Iran.

چکیده English

Oxidized alginate or alginate dialdehyde (ADA) has recently attracted a great deal of attention in the 3D bioprinting process due to the elimination or reduction of chemical cross-linking agents. In this research, alginate was oxidized with Oxidation Degrees (ODs) of 5, 7.5, and 10%, and its properties before and after the oxidation process were investigated using Fourier Transform Infrared (FTIR), Nuclear Magnetic Resonance (NMR), Gel Permeation Chromatography (GPC) tests and rheological study. Then, the bioinks containing 4% (w/v) oxidized alginate and 6% (w/v) gelatin were prepared and cross-linked according to a two-step crosslinking strategy. Then, the bioink was characterized by the printability and wettability properties. The results demonstrated that alginate oxidation reduced the molecular weight and rheological characteristics of alginate. However, the properties of the bioink (oxidized alginate-gelatin) confirmed that the properties of printability, crosslinking degree and wettability of the samples containing oxidized alginate with the OD of 5% were in the appropriate range for use as an ideal bioink.

کلیدواژه‌ها English

3D Bioprinting. Bioink

Oxidized Alginate

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