نوع مقاله : مقاله کامل پژوهشی
نویسندگان
1 دانشجوی دکتری، گروه فیزیک حالت جامد، دانشکده علوم پایه، دانشگاه مازندران، بابلسر، مازندران، ایران
2 دانشیار، گروه فیزیک حالت جامد، دانشکده علوم پایه، دانشگاه مازندران، بابلسر، مازندران، ایران
3 استادیار، گروه زیست‌شناسی سلولی و مولکولی، دانشکده علوم پایه، دانشگاه مازندران، بابلسر، مازندران، ایران
4 استادیار، گروه علوم گیاهی، دانشکده علوم پایه، دانشگاه مازندران، بابلسر، مازندران، ایران
چکیده
هدف از پژوهش حاضر ساخت نانوالیاف پلیمری کیتوزان و پلی‌وینیل‌الکل حاوی عصاره گیاه کاسنی به‌منظور ارزیابی خواص ضدباکتریایی آن است. گیاه کاسنی نیز توانایی خود را در تعدیل فعالیت و رشد باکتری‌ها با ارجاع به مقاله‌های متعدد منتشرشده آشکار می‌سازد. ازاین‌رو، عصاره گیاه کاسنی به میزان 1، 2 و 3 درصد به پیش‌ماده الیاف افزوده شد. درنهایت، ریخت‌شناسی و خاصیت ضدباکتریایی نانوالیاف حاصل بررسی شد. نتایج حاصل از میکروسکوپ الکترونی روبشی گسیل میدان (FESEM) گویای تشکیل الیاف یکنواخت است که، با افزایش میزان عصاره در نمونه، قطر الیاف از 144 نانومتر به 95 نانومتر کاهش می‌یابد. در طیف‌سنجی پراش انرژی پرتو ایکس (EDX) و آنالیز X-map، عناصر کربن، اکسیژن، نیتروژن، کلر، پتاسیم، سدیم و مس موجود در پیش‌ماده الیاف حاوی عصاره و توزیع یکنواخت آن‌ها تأیید شدند. در طیف‌سنجی مادون قرمز تبدیل فوریه (FT-IR)، گروه‌های عاملی و پیوندهای شیمیایی موجود در نانولیف‌های سنتزشده شناسایی می‌شوند. طبق نتایج مبتنی بر طیف‌سنجی فرابنفش - مرئی (UV-VIS)، یک گسیل در ناحیه فرابنفش با طول موج 263 نانومتر و دو گسیل در ناحیه فرابنفش و مرئی با طول موج‌های 270 و 372 نانومتر در طیف جذبی نانوالیاف به‌ترتیب فاقد و حاوی عصاره رؤیت شده است. بررسی خواص ضدباکتریایی در پژوهش حاضر مؤید آن است که، با افزایش میزان عصاره در نمونه، خاصیت ضدباکتریایی نانولیف‌های الکتروریسی‌شده علیه دو باکتری اشریشیاکلی و استافیلوکوکوس اورئوس افزایش می‌یابد.
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
Synthesis and Characterization of Chitosan/Poly (Vinyl Alcohol) Polymer Nanofibers Containing Chicory Extract by Electrospun Method and Evaluation of Its Antibacterial Properties
نویسندگان [English]
- Fatemeh Hedayati Tabari 1
- Habib Hamidinezhad 2
- Mohammad Karimian 3
- Ehsan Nazifi 4
1 Ph. D. Student, Department of Solid State Physics, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Mazandaran, Iran
2 Associate Professor, Department of Solid State Physics, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Mazandaran, Iran
3 Assistant Professor, Department of Cell and Molecular Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Mazandaran, Iran
4 Assistant Professor, Department of Plant Sciences, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Mazandaran, Iran
چکیده [English]
The primary objective of the current research is to produce polymeric nanofibers of chitosan and polyvinyl alcohol containing chicory plant extract and then evaluate their antibacterial effects. As remarked by numerous published articles, the chicory plant proved to be able to modulate the activity and growth of bacteria. For this reason, chicory plant extract was added to the fiber precursor in the amounts of 1, 2, and 3 %. Finally, the morphology and antibacterial properties of the resultant nanofibers were investigated. The results from Field Emission Scanning Electron Microscope (FESEM) confirmed the formation of uniform fibers whose diameters decreased from 144 nm to 95 nm with an increase in the amount of the extract in the sample. In addition, Energy Dispersive X-ray (EDX) and X-map analyses confirmed the presence of elements of carbon, oxygen, nitrogen, chlorine, potassium, sodium, and copper in the precursor of the fibers containing the extract and their uniform distribution. In this study, Fourier Transform Infrared Spectroscopy (FT-IR) helped identify the functional groups and chemical bonds in the synthesized nanofibers. According to the Ultraviolet-Visible thermometry (UV-VIS) results, one emission in the ultraviolet region with the wavelength of 263 nm and two emissions in the ultraviolet and visible regions with the wavelengths of 263 nm and 372 nm were observed in the absorption spectrum of nanofibers without and with the extract, respectively. examination of the antibacterial properties in the present study confirmed that upon increasing the amount of the extract in the sample, the antibacterial effect of the electrospun nanofibers would be intensified in exposure to both Escherichia coli and Staphylococcus aureus.
کلیدواژه‌ها [English]
- Electrospinning
- Nanofibers
- Chitosan
- Chicory
- Antibacterial
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