نوع مقاله : مقاله یادداشت پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، گروه میکروبیولوژی، دانشکده علوم زیستی، واحد فلاورجان، دانشگاه آزاد اسلامی، فلاورجان، اصفهان، ایران

2 استادیار، گروه زیست شناسی، دانشکده علوم زیستی، واحد فلاورجان، دانشگاه آزاد اسلامی، فلاورجان، اصفهان، ایران

3 استادیار، گروه میکروبیولوژی، دانشکده علوم زیستی، واحد فلاورجان، دانشگاه آزاد اسلامی، فلاورجان، اصفهان، ایران

چکیده

تحقیق حاضر با هدف بررسی اثرات نانوذرات دی اکسید تیتانیوم بر برخی از باکتریهای مقاوم به چند دارو و سپس برکبد موشهای نر نژاد ویستار انجام شد. در این مطالعه تجربی، نانوذرات بشکل کروی و بقطر nm 20، بروش هم رسوبی سنتز و اثر ضد باکتریایی آن بر چند سویه‌ باکتری با روشهای انتشار چاهک در آگار و ماکرودایلوشن ارزیابی شد. فعالیت آنزیمهای کبدیALT،ALP AST و بافت کبد در موش ها مورد بررسی قرار گرفت. داده ها با استفاده از آنالیز واریانس یکطرفه مقایسه شد. نتایج نشان داد این نانوذرات در غلظت 640 (میلی گرم بر لیتر) قادر به مهار رشد سویه‌های بالینی و استاندارد سودوموناس آئروژینوزا، انتروکوکوس فاسیوم و استافیلوکوکوس آرئوس بود . این غلظت از نانوذرات بر فعالیت آنزیمهای کبدی (AST، ALP) کاهش معنی‌داری همراه با برخی اختلالات در ساختار بافت کبد ایجاد کرد. یافته-های این پژوهش نشان داد که رشد برخی از باکتریهای بیماری‌زا تحت تأثیر این نانوذرات متوقف گردید. ولی پس از تزریق درون صفاقی به موش‌ها باعث کاهش فعالیت آنزیمهای کبدی و ناهنجاری های بافتی شد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Evaluation of the Effect of Titanium Dioxide Nanoparticles on MDR Bacteria In Vitro and Male Wistar Rats Liver In Vivo

نویسندگان [English]

  • Rozita Hosein Abbasi 1
  • Ali Noori 2
  • Monir Doudi 3

1 M. Sc. Strudent, Department of Microbiology, Faculty of Sciences, Falavarjan Branch, Islamic Azad University, Falavarjan, Isfahan, Iran

2 Assistant Professor, Department of Biology, Faculty of Sciences, Falavarjan Branch, Islamic Azad University, Falavarjan, Isfahan, Iran

3 Assistant Professor, Department of Microbiology, Faculty of Sciences, Falavarjan Branch, Islamic Azad University, Falavarjan, Isfahan, Iran

چکیده [English]

The main objective of the current study was to investigate the effects of titanium dioxide nanoparticles on some Multi-Drug Resistant (MDR) bacteria and consequently, on the liver of male Wistar rats. In this experimental study, nanoparticles in the form of spheres of 20 nm in diameter were synthesized through co-precipitation method, and their antibacterial effect on several bacterial strains was evaluated based on agar well diffusion and macrodilution methods. In addition, the activities of liver enzymes, i.e., ALT, ALP, and AST, and liver tissues in the mice were examined. The obtained data were compared using one-way ANOVA. The results confirmed the ability of these nanoparticles with the concentration of 640 (mg/L) to inhibit the growth of clinical and standard strains of Pseudomonas aeruginosa, Enterococcus faecium, and Staphylococcus aureus. In the case of the activities of liver enzymes AST and ALP, the mentioned concentration of nanoparticles caused a significant decrease in the structure of liver tissue with some disorders. The findings of this study revelaed that these nanoparticles halted the growth of some pathogenic bacteria. However, after intraperitoneal injection into the mice body, the mentioned nanoparticles reduced the activity of liver enzymes and tissue abnormalities.

کلیدواژه‌ها [English]

  • Titanium Dioxide Nanoparticles
  • Macrodilution
  • Liver Enzymes
  • Well Plate
  • Wistar Rats
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