نوع مقاله : مقاله یادداشت پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه میکروبیولوژی، دانشکده علوم زیستی، واحد فلاورجان، دانشگاه آزاد اسلامی، فلاورجان، اصفهان، ایران

2 استادیار گروه میکروبیولوژی ، دانشکده علوم زیستی ، دانشگاه آزاد اسلامی، واحد فلاورجان، ، اصفهان، ایران

3 دانشیار، گروه علوم پایه پزشکی، دانشکده دندانپزشکی، واحد اصفهان (خوراسگان)، دانشگاه آزاد اسلامی، اصفهان، اصفهان، ایران

4 استادیار، گروه زیست شناسی گیاهی، دانشکده علوم زیستی، واحد فلاورجان، دانشگاه آزاد اسلامی، فلاورجان، اصفهان، ایران

5 دانشیار، گروه علوم پایه، دانشکده علوم زیستی، واحد فلاورجان، دانشگاه آزاد اسلامی، فلاورجان، اصفهان، ایران

چکیده

با ظهور علوم جدیدی مانند فناوری نانو و هم­سویی آن با علوم مهندسی، زیست‌شناسی و پزشکی، امید به درمان بیماری‌های عفونی افزایش یافته است. از عفونت‌هایی که دامنه وسیعی از افراد جامعه را گرفتار خود کرده، عفونت زخم‌های دیابتی است که یکی از عوامل آن، باکتری استافیلوکوکوس اورئوس است. در این پژوهش، با بهره‌گیری از علم نانو و تلفیق آن با گیاه درمانگر گزنه که گیاه چندساله علفی و دارای ارزش غذایی و دارویی فراوانی است، نانوذرات اکسید روی/گزنه سنتز شد. سنتز نانوذرات اکسید روی/گزنه به روش شیمیایی مرطوب انجام شد. ارزیابی ساختاری و ریزساختاری نانوذرات اکسید روی/گزنه سنتز‌شده به‌وسیله پراش پرتو ایکس (XRD)، میکروسکوپ الکترونی عبوری (TEM) و طیف‌سنجی تبدیل فوریه مادون قرمز (FTIR) انجام شد. تأثیر ضدباکتریایی این نانوذرات بر استافیلوکوکوس اورئوس مقاوم به آنتی‌بیوتیک جدا شده از زخم دیابتی بررسی شد. عملکرد ضدباکتریایی نانوذرات اکسید روی/گزنه در برابر ایزوله‌‌های مقاوم به چند داروی (MDR) استافیلوکوکوس اورئوس، از طریق آزمون کیفی انتشار چاهک در آگار در غلظت‌های 032/0 تا 256/0 گرم/میلی لیتر نانوذرات سنتزشده انجام شد. نتایج حاصل از این پژوهش نشان داد که ‌اندازه نانوذرات سنتز شده، 100 نانومتر و ریخت‌شناسی آن‌ها دوکی‌شکل بود و بیشترین خاصیت ضدباکتریایی این نانوذرات، در غلظت 256/0 ‌گرم/میلی‌لیتر، روی جدایه‌های استافیلوکوکوس اورئوس مقاوم به آنتی‌بیوتیک، با قطر ‌هاله 20 میلی‌متر و کمترین ‌هاله عدم رشد در غلظت 032/0 ‌گرم/میلی‌لیتر مشاهده شد. با توجه به یافته‌های این پژوهش، به‌نظر می‌رسد نانوذرات اکسید روی/گزنه، فعالیت ضدباکتریایی بهتری علیه باکتری‌های استافیلوکوکوس اورئوس مقاوم به آنتی‌بیوتیک جداشده از زخم دیابتی داشتند.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Synthesis of ZnO/Urtica Dioica Nanoparticles by Wet Chemical Procedure and Investigation of Its Antibacterial Activity Against Multiple Drug Resistant (MDR) Staphylococcus aureus Isolates of Diabetic Ulcers

نویسندگان [English]

  • Samane Salemi Najafabadi 1
  • Monir Doudi 2
  • Arezoo Tahmourespour 3
  • Zahra Rezayatmand 4
  • Gholam Reza Amiri 5

1 Ph. D. Student, Department of Microbiology, Faculty of Sciences, Falavarjan Branch, Islamic Azad University, Falavarjan, Isfahan, Iran

2 Assistant Professor, Department of Microbiology, Faculty of Sciences, Falavarjan Branch, Islamic Azad University, Falavarjan, Isfahan, Iran

3 Associate Professor, Department of Basic Medical Sciences, Dental College, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Isfahan, Iran

4 Assistant Professor, Department of Plant Biology, Faculty of Sciences, Falavarjan Branch, Islamic Azad University, Falavarjan, Isfahan, Iran

5 Associate Professor, Department of Basic Sciences, Faculty of Sciences, Falavarjan Branch, Islamic Azad University, Falavarjan, Isfahan, Iran

چکیده [English]

The emergence of new sciences such as nanotechnology and its convergence with engineering, biology and medicine, it is raising hopes for the treatment of infectious diseases. One of the infections that has affected a wide range of people in the community Infection of diabetic wounds, one of the causes of which is the bacterium Staphylococcus aureus. In this study, using nanoscience and combining it with the old herbal medicine plant nettle, which is a perennial herb. And ZnO has great nutritional and medicinal value. Zinc oxide (ZnO) nanoparticles were synthesized by nettle coating. Synthesis of ZnO nanoparticles was performed by wetting the nettle plant by chemical method. Structural evaluation of minister nanoparticles of ZnO synthesized by X-Ray Diffraction (XRD), Transmission Electron Microscopy (TEM), and Fourier Transform Infrared Spectroscopy (FTIR) was performed. The antibacterial effect of this nanoparticle on antibiotic-resistant Staphylococcus aureus isolated from diabetic wounds was investigated. The antibacterial performance of ZnO/Urtica diocia nanoparticles against Staphylococcus aureus Multi Drug Resistant (MDR) isolates was evaluated by qualitative agar well diffusion test at concentrations of 0.032 to 0.256 g/ml of synthesized nanoparticles. The results of this study showed that the size of the synthesized nanoparticles was 100 nm and spindle-shaped and the highest antibacterial properties of these nanoparticles at a concentration of 0.256 g/ml on antibiotic-resistant Staphylococcus aureus isolates with a halo diameter of 20 mm and the lowest an inhibition of growth was observed at a concentration of 0.032 g/ml. According to the findings of this study it seems that ZnO/Urtica dioica nanoparticles had good antibacterial activity against antibiotic-resistant Staphylococcus aureus isolated from diabetic wounds.

کلیدواژه‌ها [English]

  • Staphylococcus aureus
  • ZnO
  • Wet chemical method
  • Urtica diocia
  • Multiple Drug Resistant (MDR)
  • Nanoparticles
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