نوع مقاله : مقاله کامل پژوهشی
نویسندگان
1 استادیار، گروه مهندسی مواد، شیمی و پلیمر، دانشگاه بین المللی امام خمینی (ره)-مرکز آموزش عالی فنی و مهندسی بوئین‌زهرا، بوئین‌زهرا، قزوین، ایران
2 استادیار، دانشکده مهندسی مواد و شیمی، مجتمع آموزش عالی فنی و مهندسی اسفراین، اسفراین، خراسان شمالی، ایران
چکیده
حضور ماکروتخلخل‌ها در داربست‌ها، علاوه بر افزایش سرعت جذب، باعث فراهم آمدن ساختاری برای رشد سلول‌های استخوانی و ایجاد استخوان جدید با ساختار کامل و یکنواخت می‌شود. در این پژوهش، پودر سیمان کلسیم فسفات، با مخلوطی از تتراکلسیم فسفات و دی‌کلسیم فسفات دوآبه، تهیه شد. برای به‌دست آوردن سوسپانسیون پایدار، پودر سیمان، با نسبت پودر به مایع 5/0 گرم بر میلی‌لیتر، با آب یون‌زدایی شده، مخلوط شد و 3 درصد وزنی دولاپیکس جامد و 4 درصد وزنی پلی‌وینیل الکل (PVA) جامد به سوسپانسیون اضافه شد. داربست‌ها، به روش ریخته‌گری انجمادی تهیه شدند. متوسط اندازه ذرات پودر سیمان، 45/5 میکرومتر تخمین زده شد. بررسی میکروسکوپ الکترونی روبشی (SEM) نشان داد که ساختار، حاوی تخلخل‌هایی در محدوده 100 تا 300 میکرومتر است و بعد از قرار‌گیری داربست‌ها در محلول شبیه به مایعات بدن، آپاتیت نانوساختار، روی دیواره تخلخل‌ها تشکیل شد. استحکام فشاری نمونه‌ها بین 2/1 تا 2/2 مگاپاسکال اندازه‌گیری شد. تکثیر سلولی نشان داد با گذشت زمان، تعداد سلول‌های شمارش شده روی سطح نمونه‌ها، تقریباً سه برابر شده است.
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
Fabrication of Nanostructured Apatite Scaffolds by Freeze-Casting Method for Bone Tissue Engineering
نویسندگان [English]
- Shokoufeh Borhan 1
- Javad Esmaeilzadeh 2
1 Assisstant Professor, Department of Materials, Chemical and Polymer Engineering, Imam Khomeini International University-Buin Zahra Higher Education Center of Engineering and Technology, Buin Zahra, Qazvin, Iran
2 Assisstant Professor, Department of Material and Chemical Engineering, Esfarayen University of Technology, Esfarayen, North Khorasan, Iran
چکیده [English]
The presence of macropores in the scaffolds, in addition to increasing the rate of adsorption, provides a structure for the growth of osteoblasts and promote the formation of new bone with a complete and uniform structure. In this study, calcium phosphate powder was prepared by mixing tetra calcium phosphate and dicalcium phosphate dihydrated. To achieve stable suspension cement powder was mixed with deionized water at powder to liquid ratio of 0.5 g/mL and 3 wt % of dispersant factor, dolapix, and 4 wt % of polyvinyl alcohol were added to suspension. Scaffolds were prepared by freeze-casting method. The average particle size of cement powder was estimated to be 5.45 μm. SEM micrographs indicate that created pores in the structure are in the range of 100-300 μm. Nanostructure apatite is formed on the wall of pores after soaking in SBF. Compressive strength of samples was measured between 1.2 to 2.2 MPa. Cell numbers on the surface of the samples are tripled over time.
کلیدواژه‌ها [English]
- Calcium Phosphate
- Freeze Casting
- Macro Porous
- Nanostructure
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