Document Type : Original Reaearch Article
Authors
1 Faculty of new Sciences and technologies, Department of Life Science Engineering, University of Tehran, Tehran, Iran
2 Department of Computer Engineering, Golestan University, Gorgan, Golestan, Iran.
3 3Department of Tissue Engineering and Applied Cell Sciences, Faculty of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran
4 Technical and Vocational Department, Gorgan, Iran
Abstract
Bone defect is one of the important challenges of medical science. Today, the synthesis of a suitable
scaffold has been developed. In this paper, new bioactive composite scaffolds based on kappa-carrageenan/fibrobin
nanofibers were synthesized and characterized for bone regeneration applications. The fibroin nanofibers were prepared
by electrospinning, then the chopped electrospun nanofibers were incorporated to Kappa-carrageenan solution (0.5%
w/v) in different ratios as a reinforcer. Finally, 3D scaffolds were fabricated by Freeze-drying. The results of scanning
electron microscopy showed the highly porous structure with the interconnected porous. The porosity percentage and
average pore size of the scaffolds were suitable for bone cells implantation and propagation. In addition, Energy
Dispersive X ray (EDS) technique has proved Precipitation of Ca-P on the surfaces. The biocompatibility evaluation of
fabricated scaffolds was done using MTT indirect assay.
Keywords
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