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Fabrication and Characterization of Properties of Silk Fibroin Scaffold Containing Ascorbic Acid-Loaded Chitosan Nanoparticles for Bone Regeneration Applications

Document Type : Original Reaearch Article

Authors

1 M. Sc. Student, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Tehran, Iran

2 Associate Professor, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Tehran, Iran

Abstract

The aim of this study was to fabricate and characterize silk fibroin scaffold containing chitosan nanoparticle loaded with ascorbic acid. Therefore, ascorbic acid- chitosan nanoparticles were fabricated using the ionic gelation method. Scanning Electron Microscopy (SEM) images and Dynamic Light Scattering (DLS) results showed that the nanoparticles are spherical with the average size of 200 nm. Then, different amounts of nanoparticles were placed in the silk fibroin solution, and finally, the scaffolds were prepared by the freeze-drying method. The effect of nanoparticle concentrations on various properties such as morphology, structural changes, water absorption, drug release, toxicity, adhesion, and alkaline phosphatase activity of MG63 cells were studied. The results of Fourier Transform Infrared Spectroscopy (FTIR) confirmed the presence of nanoparticles in the scaffold. Morphological examinations of the cross-section of the scaffold showed that all scaffolds have a porous structure with interconnected pores. The mean size of the pores and porosity percentages reduced as the nanoparticle content rose. The release of ascorbic acid in all samples started with the burst release in the first 24 hours and then continued with a controlled release for up to 14 days. Higher amounts of ascorbic acid were released from the scaffold containing more nanoparticles. Cellular studies showed that the scaffold was non-toxic and that MG63 cells adhered well onto the surface of the scaffold and the pores’ wall. Also, the proliferation and alkaline phosphatase activity of MG63 cells increased with increasing ascorbic acid amounts.

Keywords

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References
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Journal of Advanced Materials and Technologies
Volume 10, Issue 4
February 2022
Pages 25-36
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History
  • Receive Date: 09 February 2021
  • Revise Date: 01 May 2021
  • Accept Date: 06 May 2021
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