Journal of Advanced Materials and Technologies

Journal of Advanced Materials and Technologies

Fabrication and Characterization of Xanthan Film Containing Spirulina Extract with Continues Release Capability for Use in Wound Healing

Document Type : Original Reaearch Article

Authors
Afrouz Ghasemzadeh 1
Rana Imani 2
Tahura Ebrahimi 3
1 BSc Student, Biomedical Engineering Department, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran.
2 Assistant Professor, Biomedical Engineering Department, Amirkabir University of Technology (Tehran Polytechnic), Tehran. Tehran, Iran.
3 PhD Candidate, Biomedical Engineering Department, Amirkabir University of Technology (Tehran Polytechnic), Tehran. Iran
10.30501/jamt.2023.364845.1272
Abstract
Today, the most common method for wound treatment is to use engineered wound dressings. In the present study, a film wound dressing based on xanthan biopolymer, containing niosomal nanocarrier, was prepared for the local release of spirulina algae extract. Niosomal nanocarriers containing the spirulina extract were prepared using thin film hydration method. Then, a film wound dressing was produced by uniformly combining xanthan, glycerol emollient, and niosomes containing spirulina. According to scanning electron microscopy, the morphology of the niosomes were spherical with an average size of about 200 nm, and the percentage of drug loading within the niosomes was 72%. Moreover, 25% of the drug was released from the structure of the film containing niosomes within 20 days. Additionally, the wound dressing containing niosomes exhibited a 35% weight loss within 20 days. Through cytotoxicity testing conducted within 24 hours, it was revealed that up to a concentration of 200 μg/ml of the spirulina drug, cell viability remained higher than 80% compared to the control sample. In summary, the results of this study indicate that the niosomal carrier within the xanthan film structure could serve as an effective local drug delivery system for wound treatment.
Keywords
Wound Dressing
Xanthan Gum
Spirulina Extract
Controlled Release
Niosome

Subjects

  1. Abaee, A., & Madadlou, A. (2016). Niosome-loaded cold-set whey protein hydrogels. Food Chemistry, 196, 106–113. https://doi.org/10.1016/J.FOODCHEM.2015.09.037
  2. Ajji, Z., Othman, I., & Rosiak, J. M. (2005). Production of hydrogel wound dressings using gamma radiation. Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 229(3–4), 375–380. https://doi.org/10.1016/J.NIMB.2004.12.135
  3. Barbosa De Almeida, C., Catelam, K. T., Lopes Cornélio, M., Francisco, J., & Filho, L. (2010). Morphological and Structural Characteristics of Zein Biofilms with Added Xanthan Gum. Food Technology and Biotechnology, 48(1), 19–27. https://www.researchgate.net/publication/41764895
  4. Behyari, M., Imani, R., & Keshvari, H. (2021). Evaluation of Silk Fibroin Nanofibrous Dressing Incorporating Niosomal Propolis, for Potential Use in Wound Healing. Fibers and Polymers, 22(8), 2090–2101. https://doi.org/10.1007/s12221-021-0973-2
  5. Bilanovic, D., Starosvetsky, J., & Armon, R. H. (2016). Preparation of biodegradable xanthan–glycerol hydrogel, foam, film, aerogel and xerogel at room temperature. Carbohydrate Polymers, 148, 243–250. https://doi.org/10.1016/J.CARBPOL.2016.04.058
  6. Brunchi, C. E., Bercea, M., Morariu, S., & Dascalu, M. (2016). Some properties of xanthan gum in aqueous solutions: effect of temperature and pH. Journal of Polymer Research, 23(7), 1–8.  https://doi.org/10.1007/s10965-016-1015-4
  7. Bueno, V. B., & Petri, D. F. S. (2014). Xanthan hydrogel films: Molecular conformation, charge density and protein carriers. Carbohydrate Polymers, 101(1), 897–904. https://doi.org/10.1016/J.CARBPOL.2013.10.039
  8. Choi, J. Il, Kim, M. S., Chun, G. Y., & Shin, H. S. (2017). Spirulina extract-impregnated alginate-PCL nanofiber wound dressing for skin regeneration. Biotechnology and Bioprocess Engineering, 22(6), 679–685. https://doi.org/10.1007/s12257-017-0329-3
  9. de Morais Lima, M., Carneiro, L. C., Bianchini, D., Dias, A. R. G., Zavareze, E. D. R., Prentice, C., & Moreira, A. D. S. (2017). Structural, Thermal, Physical, Mechanical, and Barrier Properties of Chitosan Films with the Addition of Xanthan Gum. Journal of Food Science, 82(3), 698–705. https://doi.org/10.1111/1750-3841.13653
  10. Desmet, E., Van Gele, M., & Lambert, J. (2017). Topically applied lipid- and surfactant-based nanoparticles in the treatment of skin disorders. Expert Opinion on Drug Delivery, 14(1), 109–122. https://doi.org/10.1080/17425247.2016.1206073
  11. Dhivya, S., Padma, V. V., & Santhini, E. (2015). Wound dressings-A review. BioMedicine (Netherlands), 5(4), 24–28. https://doi.org/10.7603/S40681-015-0022-9
  12. Frykberg, R. G., & Banks, J. (2015). Challenges in the Treatment of Chronic Wounds. Advances in Wound Care, 4(9), 560–582. https://doi.org/10.1089/WOUND.2015.0635
  13. García-Ochoa, F., Santos, V. E., Casas, J. A., & Gómez, E. (2000). Xanthan gum: production, recovery, and properties. Biotechnology Advances, 18(7), 549–579. https://doi.org/10.1016/S0734-9750(00)00050-1
  14. Ge, X., Wei, M., He, S., & Yuan, W. E. (2019). Advances of Non-Ionic Surfactant Vesicles (Niosomes) and Their Application in Drug Delivery. Pharmaceutics 11(2), 55. https://doi.org/10.3390/PHARMACEUTICS11020055
  15. Gunes, S., Tamburaci, S., Dalay, M. C., & Gurhan, I. D. (2017). In vitro evaluation of Spirulina platensis extract incorporated skin cream with its wound healing and antioxidant activities. Pharmaceutical Biology, 55(1), 1824–1832. https://doi.org/10.1080/13880209.2017.1331249
  16. Jung, S. M., Min, S. K., Lee, H. C., Kwon, Y. S., Jung, M. H., & Shin, H. S. (2016). Spirulina-PCL Nanofiber Wound Dressing to Improve Cutaneous Wound Healing by Enhancing Antioxidative Mechanism. Journal of Nanomaterials, 2016. https://doi.org/10.1155/2016/6135727
  17. Khoee, S., & Yaghoobian, M. (2017). Niosomes: a novel approach in modern drug delivery systems. Nanostructures for Drug Delivery, 207–237. https://doi.org/10.1016/B978-0-323-46143-6.00006-3
  18. Kokabi, M., Sirousazar, M., & Hassan, Z. M. (2007). PVA–clay nanocomposite hydrogels for wound dressing. European Polymer Journal, 43(3), 773–781. https://doi.org/10.1016/J.EURPOLYMJ.2006.11.030
  19. Laracuente, M. L., Yu, M. H., & McHugh, K. J. (2020). Zero-order drug delivery: State of the art and future prospects. Journal of Controlled Release, 327, 834–856. https://doi.org/10.1016/J.JCONREL.2020.09.020
  20. Malektaj, H., Imani, R., & Siadati, M. H. (2021). Study of injectable PNIPAAm hydrogels containing niosomal angiogenetic drug delivery system for potential cardiac tissue regeneration. Biomedical Materials, 16(4), 045031. https://doi.org/10.1088/1748-605X/abdef8
  21. Mitragotri, S. (2005). Healing sound: the use of ultrasound in drug delivery and other therapeutic applications. Nature Reviews Drug Discovery 2005 4:3, 4(3), 255–260. https://doi.org/10.1038/nrd1662
  22. Nishinari, K., Yamatoya, K., Shirakawa, M., Phillips, G. O., & Williams, P. A. (2000). Handbook of hydrocolloids. Woodhead Publ, 247-267.
  23. Nour, S., Imani, R., & Sharifi, A. M. (2022). Angiogenic Effect of a Nanoniosomal Deferoxamine-Loaded Poly (vinyl alcohol)-Egg White Film as a Promising Wound Dressing. ACS Biomaterials Science and Engineering, 8(8), 3485–3497. https://pubs.acs.org/doi/10.1021/acsbiomaterials.2c00046
  24. Nur, P., Syarina, A., Karthivashan, G., Abas, F., Arulselvan, P., & Fakurazi, S. (2015). Wound Healing Potential of Spirulina Platensis Extracts On Human Dermal Fibroblast Cells. EXCLI Journal, 14, 385–393. https://doi.org/10.17179/excli2014-697
  25. Pando, D., Matos, M., Gutiérrez, G., & Pazos, C. (2015). Formulation of resveratrol entrapped niosomes for topical use. Colloids and Surfaces B: Biointerfaces, 128, 398–404. https://doi.org/10.1016/J.COLSURFB.2015.02.037
  26. Patias, L. D., Maroneze, M. M., Siqueira, S. F., de Menezes, C. R., Zepka, L. Q., & Jacob-Lopes, E. (2018). Single-Cell Protein as a Source of Biologically Active Ingredients for the Formulation of Antiobesity Foods. Alternative and Replacement Foods, 17, 317–353. https://doi.org/10.1016/B978-0-12-811446-9.00011-3
  27. Rahman, S. A., Abdelmalak, N. S., Badawi, A., Elbayoumy, T., Sabry, N., & Ramly, A. E. (2015). Formulation of tretinoin-loaded topical proniosomes for treatment of acne: in-vitro characterization, skin irritation test and comparative clinical study. Drug Delivery, 22(6), 731–739. https://doi.org/10.3109/10717544.2014.896428
  28. Richmond, N. A., Lamel, S. A., Davidson, J. M., Martins-Green, M., Sen, C. K., Tomic-Canic, M., Vivas, A. C., Braun, L. R., & Kirsner, R. S. (2013). US–National Institutes of Health-funded research for cutaneous wounds in 2012. Wound Repair and Regeneration, 21(6), 789–792. https://doi.org/10.1111/WRR.12099
  29. Saghazadeh, S., Rinoldi, C., Schot, M., Kashaf, S. S., Sharifi, F., Jalilian, E., Nuutila, K., Giatsidis, G., Mostafalu, P., Derakhshandeh, H., Yue, K., Swieszkowski, W., Memic, A., Tamayol, A., & Khademhosseini, A. (2018). Drug delivery systems and materials for wound healing applications. Advanced Drug Delivery Reviews, 127, 138–166. https://doi.org/10.1016/J.ADDR.2018.04.008
  30. Sehgal, P. K., Sripriya, R., Senthilkumar, M., & Rajendran, S. (2019). Drug delivery dressings. Advanced Textiles for Wound Care, 261–288. https://doi.org/10.1016/B978-0-08-102192-7.00009-6
  31. Shalviri, A., Liu, Q., Abdekhodaie, M. J., & Wu, X. Y. (2010). Novel modified starch–xanthan gum hydrogels for controlled drug delivery: Synthesis and characterization. Carbohydrate Polymers, 79(4), 898–907. https://doi.org/10.1016/J.CARBPOL.2009.10.016
  32. Sharma, V., Anandhakumar, S., & Sasidharan, M. (2015). Self-degrading niosomes for encapsulation of hydrophilic and hydrophobic drugs: An efficient carrier for cancer multi-drug delivery. Materials Science and Engineering: C, 56, 393–400. https://doi.org/10.1016/J.MSEC.2015.06.049
  33. Shehata, T. M., Ibrahim, M. M., & Elsewedy, H. S. (2021). Curcumin Niosomes Prepared from Proniosomal Gels: In Vitro Skin Permeability, Kinetic and In Vivo Studies. Polymers, 13(5), 791. https://doi.org/10.3390/POLYM13050791
  34. Shemesh, M., & Zilberman, M. (2014). Structure–property effects of novel bioresorbable hybrid structures with controlled release of analgesic drugs for wound healing applications. Acta Biomaterialia, 10(3), 1380–1391. https://doi.org/10.1016/J.ACTBIO.2013.11.025
  35. Shinde, S., Lavale, S. A., & Nagare, K. (2018). ‘Spirulina’as an Additive for Better Nutrition. J. Curr. Microbiol. App. Sci, 7(5), 143-147. https://www.ijcmas.com/7-5-2018/Supriya%20Shinde,%20et%20al.pdf
  36. Swinehart, D. F. (1962). The Beer-Lambert Law. Journal of Chemical Education, 39(7), 333–335. https://doi.org/10.1021/ED039P333
  37. Vimala, K., Mohan, Y. M., Varaprasad, K., Redd, N. N., Ravindra, S., Naidu, N. S., & Raju, K. M. (2011). Fabrication of Curcumin Encapsulated Chitosan-PVA Silver Nanocomposite Films for Improved Antimicrobial Activity. Journal of Biomaterials and Nanobiotechnology, 02(01), 55–64. https://doi.org/10.4236/JBNB.2011.21008
  38. White, R. (2005). Evidence for atraumatic soft silicone wound dressing use. Wounds UK, 1(3), 104-109. https://wounds-uk.com/wp-content/uploads/2023/02/content_9034.pdf
  39. Zannis, J., Angobaldo, J., Marks, M., Defranzo, A., David, L., Molnar, J., & Argenta, L. (2009). Comparison of fasciotomy wound closures using traditional dressing changes and the vacuum-assisted closure device. Annals of Plastic Surgery, 62(4), 407–409. https://doi.org/10.1097/SAP.0B013E3181881B29
Journal of Advanced Materials and Technologies
Volume 12, Issue 4
Winter 2024
Pages 1-14

  • Receive Date 09 April 2023
  • Revise Date 15 July 2023
  • Accept Date 26 December 2023