Document Type : Original Reaearch Article
Authors
1 Ph. D. Candidate, Department of Nanotechnology and Advanced Materials, Materials and Energy Research Center, Karaj, Iran.
2 Professor, Department of Nanotechnology and Advanced Materials, Materials and Energy Research Center, Karaj, Iran.
3 Associate Professor, Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran, Iran.
4 Ph.D, Faculty of biological sciences, Kharazmi University, Tehran, Iran.
Abstract
Oxidized alginate or alginate dialdehyde (ADA) has recently attracted a great deal of attention in the 3D bioprinting process due to the elimination or reduction of chemical cross-linking agents. In this research, alginate was oxidized with Oxidation Degrees (ODs) of 5, 7.5, and 10%, and its properties before and after the oxidation process were investigated using Fourier Transform Infrared (FTIR), Nuclear Magnetic Resonance (NMR), Gel Permeation Chromatography (GPC) tests and rheological study. Then, the bioinks containing 4% (w/v) oxidized alginate and 6% (w/v) gelatin were prepared and cross-linked according to a two-step crosslinking strategy. Then, the bioink was characterized by the printability and wettability properties. The results demonstrated that alginate oxidation reduced the molecular weight and rheological characteristics of alginate. However, the properties of the bioink (oxidized alginate-gelatin) confirmed that the properties of printability, crosslinking degree and wettability of the samples containing oxidized alginate with the OD of 5% were in the appropriate range for use as an ideal bioink.
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Main Subjects
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