Journal of Advanced Materials and Technologies

Journal of Advanced Materials and Technologies

The Effect of Adding Different Percentages of γ-Glycidoxypropyltrimethoxysilane Cross-linker on the Properties of Freeze-dried Gelatin/Chitosan Scaffolds upon Introducing an Optimum Condition to Cross-link the Chitosan/Gelatin Scaffolds in Bone Tissue Engineering

Document Type : Original Reaearch Article

Authors
Fatemeh Banafatizadeh 1
Ali Zamanian 2
1 Ph. D. Candidate, Biomaterials Research Group, Department of Nanotechnology and Advanced Materials, Materials and Energy Research Center, Karaj, Iran
2 Professor, Biomaterials Research Group, Department of Nanotechnology and Advanced Materials, Materials and Energy Research Center, Karaj, Iran
10.30501/jamt.2022.347635.1229
Abstract
Use of γ-glycidoxypropyltrimethoxysilane (GPTMS) enhances the stability and strength of hydrogels and improves the bioactivity and cellular performance of the scaffolds in bone applications. Given that one of the leading factors that affects the properties of the scaffold is the percentage of the cross-linking agent, chitosan and gelatin scaffolds with different percentages of GPTMS were prepared and evaluated through freeze-drying method. The results from Scanning Electron Microscope (SEM) confirmed the achievement of porous scaffolds with open pores and upon increasing the amount of cross-linking agent, the size of the pores reached approximately 290 microns. The results of the infrared spectrum showed the interaction among the polymers and process of cross-linking with the formation of silane groups. In this study, upon increasing the amount of the transverse bonding agent, the contact angle decreased, the optimal contact angle of the sample with 75 (weight percent) GPTMS reached 60.7 ± 3.5 degrees, and the percentages of both swelling and degradation ultimately decreased. It should be noted that the mechanical properties were improved by adding GPTMS up to 1590 ± 267 kPa. According to the findings of this study, application of GPTMS led to an enhancement in the bioactivity properties and deposition of the hydroxyapatite layer, and the X-ray diffraction pattern confirmed this claim. The obtained results support the hypothesis that gelatin-chitosan scaffolds with 75 (wt %) GPTMS seem to be the best samples for use in bone tissue engineering.
Keywords
Gelatin
Chitosan
Cross-linker
Bone Tissue Engineering
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Journal of Advanced Materials and Technologies
Volume 12, Issue 2
Summer 2023
Pages 39-54

  • Receive Date 05 July 2022
  • Revise Date 10 September 2022
  • Accept Date 14 September 2022