Journal of Advanced Materials and Technologies

Journal of Advanced Materials and Technologies

Preparation and Characterization of Composite Bone Scaffold Based on Polycaprolactone and Sulfonated Silk Fibroin Nanofibers

Document Type : Original Reaearch Article

Authors
Negin Vahedi 1
Jhamak Nourmohammadi 2
Niloofar Pahlevani 3
Sahar Nakhostin Hosseini 1
1 M. Sc. Student, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Tehran, Iran
2 Associate Professor, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Tehran, Iran
3 M. Sc. Student, Faculty of Technology and Engineering, Central Tehran Branch, Islamic Azad University, Tehran, Tehran, Iran
10.30501/jamt.2021.250243.1128
Abstract
The objective of this study was to fabricate and characterize the characteristics of polycaprolactone-sulfonated silk fibroin nanofibrous scaffold for bone tissue engineering applications. Thus, after extraction of silk fibroin (SF) from from Bombyx mori cocoons, silk fibroin (SF) was sulfonated using chlorosulfonic acid and pyridine. Afterwards, a certain amount of sulfonated SF was mixed with polycaprolactone (PCL) solution, and then electrospinning was done using 11 kV high voltage and feeding rate 0.4 mL/h. Various characterization tests were applied to analyze such items such as the structure, chemical composition, bioactivity, cellular attachment, and viability. Fourier transform infrared spectroscopy analyses proved the successful incorporation of sulfate and sulfonate groups in SF structure. The scanning electron microscope shows the formation of continuous and beadless fibers. The average fiber diameter in polycaprolactone nanofibers reduced from 244 nm to 138 nm with the addition of sulfonated SF. Moreover, the water uptake of PCL nanofibers improved from 12.4 % to 167 % after the addition of sulfonated silk fibroin to polycaprolactone. The results of bioactivity and cell culture experiments indicated that sulfonated SF promotes apatitic calcium phosphate deposition and also enhances cellular attachment and viability of PCL nanofibers.
Keywords
Polycaprolactone
Sulfonated Silk Fibroin
Calcium Phosphate Deposition
Bone Scaffold

Subjects

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Journal of Advanced Materials and Technologies
Volume 10, Issue 1
Spring 2021
Pages 75-84

  • Receive Date 26 September 2020
  • Revise Date 04 November 2020
  • Accept Date 22 August 2021