Journal of Advanced Materials and Technologies

Journal of Advanced Materials and Technologies

Formulation, Characterization and Evaluation of Quercetin-loaded Chitosan-functionalized Mesoporous Silica for Targeted Drug Delivery in Cancer Treatment

Authors
Navid Ahmadi Nasab 1
Hassan Hassani Kumleh 2
Shahram Teimourian 3
Mojtaba Beygzadeh 4
Mahmood Kazemzad 4
1 University of Guilan, University Campus 2, Department of Biotechnology, Rasht, Iran
2 University of Guilan, Department of Biotechnology, Faculty of Agricultural Sciences, Rasht, Iran
3 Iran University of Medical Sciences, Department of Medical genetics, Tehran, Iran
4 Materials and Energy Research Center, Department of Energy, Karaj, Iran
10.30501/jamt.2637.70346
Abstract
Quercetin is a safe herbal metabolism which has effective role on inhibition of tumor cells and growth of many types of cancers. In this study to improve the efficiency and bioavailability of Quercetin molecule, Chitosan-functionalized nanoporous mesoporous Silica was used as a pH-sensitive biopolymer. For this purpose, at first nanoporous Mesoporous silica was synthesized by sol-gel method; then Chitosan layer was coated on nanoporous of silica using GPTMS in an acidic medium. Different analysis methods such as FESEM, BET and FTIR were used to characterize the synthesized nanocarrier. FESEM and BET analysis results demonstrated nanometric sizes of particles and porosities. FTIR analysis picks showed successful drug loading on nanocarrier. On the other hand, calculation of drug loading content and encapsulation efficiency in acidic and physiologic pH, illustrated that nanocarrier has suitable performance and stability for drug loading and release. Cytotoxicity of as-drug formulation in different concentrations was investigated by MTT assay on HeLa cells. The results showed that cytotoxicity of as-drug formulation increased in comparison to free drug and IC50 value of HeLa cells decreased from 58 µM for free Quercetin to 36 µM for loaded Quercetin. Eventually this study showed Chitosan-functionalized Mesoporous Silica nanocarrier could be considered as a smart and biocompatible drug delivery system to carry Quercetin drug for cancer cells treatment.
Keywords
Smart Drug Delivery System
Nanocarriers
Flavonoid
Cytotoxicity
Cancer treatment

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  • Receive Date 06 February 2017
  • Revise Date 19 February 2017
  • Accept Date 25 February 2017