Flame Annealed Micro-Carbon Fiber Electrode Modified with Mild Oxidized Water-Dispersible Graphene Oxide for Electrochemical Sensing of Dopamine in the presence of Uric Acid

Alizadeh, Vali; Hosseinifard, Mojtaba

doi:10.30501/jamt.2026.536005.1334

Flame Annealed Micro-Carbon Fiber Electrode Modified with Mild Oxidized Water-Dispersible Graphene Oxide for Electrochemical Sensing of Dopamine in the presence of Uric Acid

Document Type : Original Reaearch Article

Authors

Vali Alizadeh ¹

Mojtaba Hosseinifard ²

¹ Assistant Professor, Department of Petroleum, Faculty of Engineering, University of Garmsar, Garmsar, Iran.

² Associate Professor, Department of Energy, Materials and Energy Research Center, Karaj, Iran.

10.30501/jamt.2026.536005.1334

Abstract

In this research, a micro carbon fiber electrode (MCFE) was activated using a simple flame annealing method and subsequently modified with mildly oxidized graphene oxide (M-GO) to develop an inexpensive and sensitive electrochemical sensor for dopamine (DA) detection. Observations indicate that flame annealing increases surface roughness, enhances electrochemical activity, and improves the electrode’s affinity for M-GO adsorption, resulting in a more sensitive DA sensor. The electrochemical properties and morphology of the modified electrode were characterized using electrochemical techniques, including cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), differential pulse voltammetry (DPV), and scanning electron microscopy (SEM). Electrochemical investigations revealed that the M-GO-modified flame-annealed micro carbon fiber electrode (FA-MCFE/M-GO) exhibits high sensitivity, a wide linear range, and a detection limit of 20 nM for DA determination. Another notable feature of this sensor is its ability to eliminate the interfering effects of uric acid (UA) during dopamine detection, which represents one of the primary challenges in the electrochemical analysis of DA in biological samples. The results demonstrate that using M-GO as a surface modifier for the carbon fiber microelectrode provides an effective and cost-efficient strategy for fabricating high-performance electrochemical sensors. This sensor shows substantial potential for diagnostic and clinical applications in measuring DA in the presence of interfering substances.

Keywords

Flame Annealing Activation

Carbon Fiber Microelectrode

Dopamine

Uric Acid

DPV

Subjects

Nanomaterials and nanostructures

1. Chen, J., Xia, F., Ding, X., & Zhang, D. (2024). Universal Covalent Grafting Strategy of an Aptamer on a Carbon Fiber Microelectrode for Selective Determination of Dopamine In Vivo. Analytical Chemistry, 96(25), 10322-10331. https://doi.org/10.1021/acs.analchem.4c01167

2. Dreye, D. R., Park, S., Bielawski, C.W. &Ruoff, R. S. (2010). The chemistry of graphene oxide. Chemical Society Reviews, 39(1), 228–240. https://doi.org/10.1039/B917103G

3. Ekrami-Kakhki, M-S., Farzaneh, N., & Abbasi, S. (2019).Improved Electrocatalytic Activity of Pt-SrCoO3-δ Nanoparticles Supported on Reduced Graphene Oxide for Methanol Electrooxidation. Journal of Advanced Materials and Technology,8(3), 49-58.https://doi.org/10.30501/jamt.2019.93884

4. Huang, Q., Zhang, H., Hu, S., Li, F., Weng, W., Chen, J., Wang, Q., He, Y., Zhang, W., & Bao, X. (2014). A sensitive and Reliable Dopamine Biosensor Was Developed Based on the Au@ Carbon Dots-Chitosan Composite Film. Biosensors and. Bioelectronics, 52, 277−280.https://doi.org/10.1016/j.bios.2013.09.003

5. Kharazmi, Sh., & Alamdari, S. (2024). Electrical and Mechanical Performance of Chitosan Films Enhanced by Graphene Oxide and Silver Nanocomposites: Synthesis, Characterization, and Comparative Analysis. Advanced Ceramics Progress, 10(2), 32-39. https://doi. org/10.30501/acp.2024.473596.1159

6. Madhurantakam, S., Karnam, J.B., Brabazon, D., Takai, M., Ahad, I.U., Balaguru Rayappan, J.B., & Krishnan, U.M. (2020). “Nano”: An Emerging Avenue in Electrochemical Detection of Neurotransmitters. ACS Chemical Neuroscience, 11(24), 4024-4047. https://doi.org/10.1021/acschemneuro.0c00355

7. Mgenge, L., Saha, C., Kumari, P., Ghosh, S. K., Singh, H., & Mallick, K. (2025). Electrochemical sensing of dopamine using nanostructured silver chromate: Development of an IoT-integrated sensor. Analytical Biochemistry, 698, 115726.https://doi.org/10.1016/j.ab.2024.115726

8. Rosenwald, S.E., Nowall, W.B., Dontha, N., & Kuhr, W.G. (2000). Laser Interface Pattern Ablation of a Carbon Fiber Microelectrode: Biosensor Signal Enhancement after Enzyme Attachment. Analytical chemistry, 72(20), 4914-4920. https://doi.org/10.1021/ac000442t

9. Seven, F., Golcez, T., & Sen, M. (2020). Nanoporous carbon-fiber microelectrodes for sensitive detection of H₂O₂ and dopamine. Journal of Electroanalytical Chemistry, 864, 114104. https://doi.org/10.1016/j.jelechem.2020.114104

10. Strand, A.M., & Venton, B.J. (2008). Flame Etching Enhances the Sensitivity of Carbon-Fiber Microelectrodes. Analytical Chemistry, 80(10), 3708-3715. https://doi.org/10.1021/ac8001275

11. Takmakov, P., Zachek, M.K., Keithley, R.B., Walsh, P.L., Donley, C., McCarty, G.S., & Wightman, R.M., (2010). Carbon Microelectrode with a Renewable Surface. Analytical Chemistry, 82(5), 2020-2028.https://pubs.acs.org/doi/10.1021/ac902753x

12. Valikchalia, F. G., Rahimnejad, M., Ramiarb, A., & Ezojic, M. (2022). Diagnostics Devices for Improving the World: μPADs Integrated with Smartphone for Colorimetric Detection of Dopamine. International Journal of Engineering, 35(9), 1723-1727.https://doi.org/ 10.5829/ije.2022.35.09C.07

13. Xu. Y., Sheng, K., Li, C., & Shi, G. (2011). Highly Conductive Chemically Converted Graphene Prepared from Mildly Oxidized Graphene Oxide. Journal of Material Chemistry,21(20), 7376-7380.https://doi.org/10.1039/C1JM10768B

14. You, X., Yang, S., Li, J., Deng, Y., Dai, L., Peng, X., Huang, H., Sun, J., Wang, G., He, P., Ding, G., & Xie., X. (2017).Green and Mild Oxidation: An Efficient Strategy toward Water-Dispersible Graphene. ACS Applied Materials & Interfaces, 9(3), 2856-2866. https://doi.org/10.1021/acsami.6b13703