مواد و فناوری‌های پیشرفته

مواد و فناوری‌های پیشرفته

تولید و مشخصه‌یابی هیدروژل حساس به دما و قابل‌تزریق پلارونیک F127 برای کاربرد رهایش هدفمند دارو در درمان سرطان

نوع مقاله : مقاله کامل پژوهشی

نویسندگان
مهتاب فرزانه 1
سید علی حسن زاده تبریزی 2
نادر مختاریان 3
1 کارشناسی ارشد، دانشکده مهندسی ساخت و فناوری‌های صنعتی، واحد نجف‌آباد، دانشگاه آزاد اسلامی، نجف‌آباد، اصفهان، ایران
2 استاد، دانشکده مهندسی ساخت و فناوری‌های صنعتی، واحد نجف‌آباد، دانشگاه آزاد اسلامی، نجف‌آباد، اصفهان، ایران
3 استادیار، گروه مهندسی شیمی، واحد شهرضا، دانشگاه آزاد اسلامی، شهرضا، اصفهان، ایران
10.30501/jamt.2025.504800.1318
چکیده
در این مطالعه، یک هیدروژل قابل‌تزریق از جنس Pluronic F127 حاوی دوکسوروبیسین (DOX) با خواص حساسیت به دما برای دارورسانی در محل و موضعی تهیه شد. هیدروژل به «روش سرد» تهیه شد و تأثیر غلظت هیدروژل بر دمای ژل شدن و ویسکوزیته و تأثیر دما بر میزان رهایش دارو بررسی شد. غلظت‌های گوناگون هیدروژل F127 از نظر ویسکوزیته و سایر ویژگی‌ها و رهایش دوکسوروبیسین در دمای طبیعی بافت بدن (37 درجه‌ی سلسیوس) و در دمای تومور سرطانی (۴3 درجه‌ی سلسیوس) بررسی شد. غلظت بهینه‌ی هیدروژل F127 معادل ۲۰ درصد وزنی‌حجمی انتخاب و خواص رئولوژیکی و رهایش داروی آن بررسی شد. هیدروژل F127 با مادون قرمز تبدیل فوریه (FTIR) و پراش پرتو ایکس (XRD) مشخصه‌یابی شد و با استفاده از میکروسکوپ الکترونی روبشی نشر میدانی با تابش پراکندگی انرژی (FE-SEM/EDS) بررسی شد. نتایج آزمون رهایش دارو حاکی از رهایش موفق دوکسوروبیسین در هر دو دما بود. نتایج نشان می‌دهد که مقدار دوکسوروبیسین آزادشده با افزایش دما حدود ۱۸-۲۰ درصد افزایش می‎یابد که برای درمان تومورهای بدخیم مهم است. نتایج آزمون MTT همچنین نشان می‌دهد که هیدروژل حاوی دارو باعث کاهش زنده ماندن سلول‏های سرطانی می‎شود و تنها 32 درصد از سلول‎ها پس از 72 ساعت در محیط کشت زنده مانده‎اند.
کلیدواژه‌ها
هیدروژل
دوکسوروبیسین
F127
سرطان
حساس به دما

موضوعات

عنوان مقاله English

Preparation and Characterization of an Injectable Thermosensitive Pluronic F127 Hydrogel for Targeted Drug Delivery in Cancer Treatment

نویسندگان English

Mahtab Farzaneh 1
Sayed Ali Hassanzadeh-Tabrizi 2
Nader Mokhtarian 3
1 Master of Biomedical Engineering Graduate, Institute of Manufacturing Engineering and Industrial Technologies, Na.C, Islamic Azad University, Najafabad, Isfahan, Iran.
2 Professor, Institute of Manufacturing Engineering and Industrial Technologies, Na.C, Islamic Azad University, Najafabad, Isfahan, Iran.
3 Assistant Professor, Department of Chemical Engineering, Shahreza Branch, Islamic Azad University, Shahreza, Isfahan, Iran.
چکیده English

In this study, an injectable hydrogel made from Pluronic F127 containing Doxorubicin (DOX), with thermosensitive properties for localized drug delivery, was prepared using the cold method. The effects of hydrogel concentration on gelation temperature and viscosity, as well as the effect of temperature on drug release rate, were investigated. Different concentrations of F127 hydrogel were evaluated for viscosity and other characteristics, and Doxorubicin release was studied at body temperature (37℃) and tumor temperature (43℃). The optimal concentration of F127 hydrogel was selected as 20% w/v, and its rheological and drug release properties were examined. The hydrogel was characterized using Fourier Transform Infrared (FTIR) and X-Ray Diffraction (XRD), and examined through Field Emission Scanning Electron Microscopy with Electrophoretic Light Scattering (FE-SEM/EDS). The drug release assay results indicated successful Doxorubicin release at both temperatures. The release increased by 18-20% with temperature rise, which is significant for malignant tumor treatment. The MTT assay results showed that the drug-containing hydrogel reduced cancer cell viability, with only 32% of the cells surviving after 72 hours in culture.

کلیدواژه‌ها English

Hydrogel
Doxorubicin
F127
Cancer
Temperature Sensitive
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مواد و فناوری‌های پیشرفته
دوره 14، شماره 1
بهار 1404
صفحه 28-42

  • تاریخ دریافت 17 بهمن 1403
  • تاریخ بازنگری 22 اسفند 1403
  • تاریخ پذیرش 05 خرداد 1404