ارزیابی خواص فیزیکی و مکانیکی داربست نانوکامپوزیتی الکتروریسی شده پلی‌ɛ-کاپرولاکتون-ژلاتین/ نانولوله‌کربنی چنددیواره

نوع مقاله: یادداشت پژوهشی

نویسندگان

1 گروه مهندسی پزشکی- بیومتریال، دانشکده علوم و فنون نوین، دانشگاه تهران، تهران، ایران

2 گروه بیومتریال و مهندسی بافت، دانشکده فناوری های نوین علوم پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

چکیده

داربست نانوفیبری PCL/gelatin با نسبت وزنی 70:30 و داربست نانوکامپوزیتی 70:30 PCL/gelatin  حاوی 5/0 درصد وزنی نانولوله کربنی چند‌دیواره عامل‌دار شده با گروه کربوکسیلfunctionalized by carboxyl) ) به روش الکتروریسی تهیه شدند. مورفولوژی، خواص فیزیکی و مکانیکی داربست‌ها به وسیله میکروسکوپ الکترونی، طیف‌سنجی مادون قرمز، زاویه تماس آب و استحکام کششی مورد ارزیابی قرار گرفت. داربست حاوی MWNTs به علت افزایش رسانایی محلول و نحوه قرار گرفتن MWNTs در نانو‌الیاف پلیمری، از توزیع قطر الیاف و میانگین قطر الیاف مناسب‌تری نسبت به داربست فاقد MWNTs برخوردار است. حضور MWNTs هیچ‌گونه اثر مخربی بر روی درصد تخلخل سطح داربست‌ نداشته و درصد تخلخل در سطح داربست‌ها بیش از 80% است. بر اساس طیف‌سنجی مادون قرمز‌، برهم-کنش میان گروه آمین ژلاتین و گروه‌ کربوکسیل MWNTs، می‌تواند به‌طور مستقیم بر روی خواص مکانیکی تاثیر‌گذار باشد. افزودن MWNTs به داربست نانوفیبری70:30 PCL/gelatin موجب کاهش قابل توجه زاویه تماس آب و افزایش قابل ملاحظه میانگین استحکام کششی تا حدود هفت برابر نسبت به داربست فاقد MWNTs شده است. بهبود خواص مکانیکی داربست را می‌توان به علت استحکام ذاتی بالای MWNTs، قرار‌گیری آن در طول الیاف پلیمری و پراکندگی مناسب آن در زمینه پلیمری دانست. داربست PCL-gelatin/0.5%wt.MWNT می‌تواند یک داربست مناسب برای کاربردهای مهندسی بافت باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of physical and mechanical properties of electrospinning nanocomposite scaffolds poly ɛ-caprolactone-gelatin/multi walled carbon nanotube

نویسندگان [English]

  • Parisa Zadehnajar 1
  • Saeed Karbasi 2
  • babak Akbari 1
  • Mohamad Hossein Mirmusavi 2
1 Department of Biomaterials, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran
2 Department of Biomaterials and Tissue Engineering, School of Advance Technology in Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
چکیده [English]

The poly (ɛ-caprolactone)/gelatin nanofibrous scaffolds with weight ratio of 70:30 and PCL/gelatin 70:30 nanocomposite scaffold containing 0.5%wt. MWNTs (PCL-gelatin/0.5%wt.MWNT) were fabricated through electrospinning. The morphology, physical and mechanical property of the scaffolds was evaluated through SEM, FTIR-ATR, water contact angle and tensile strength test. The scaffold containing 0.5%wt. MWNTs had the best average and distribution of fibers diameter in comparison with PCL/gelatin scaffold due to the increased conductivity of the solution and the alignment of the MWNTs in the nanofibers. The presence of MWNTs did not have any reverse effect on the porosity of the scaffolds and the porosity percentage of the scaffolds was more than 80%. According to FTIR spectra there was a connection between gelatin amine group and MWNTs carboxylic group that could affect the mechanical properties directly. Adding 0.5%wt. MWNTs to the PCL/gelatin scaffold decreased contact angle and lead to an increase in mean tensile strength about 7 times in comparison with scaffold without MWNTs. The enhancement of the mechanical properties of the scaffold can be seen due to the inherent strength of MWNTs, the position of the MWNTs in the polymer nanofibers and the optimal dispersion in the polymer matrix. PCL-gelatin/0.5%wt.MWNT scaffold can be an appropriate scaffold for tissue engineering applications.

کلیدواژه‌ها [English]

  • nanocomposite scaffold
  • Tissue engineering
  • Carbon Nanotube
  • Electrospinning

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